Secretion of human epidermal growth factor by Corynebacterium glutamicum
Secretion of human epidermal growth factor by Corynebacterium glutamicum
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DOI：
10.1111/j.1472-765x.2005.01802.x
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发表时间：
2006-01-01
影响因子：
2.4
通讯作者：
Kikuchi, Y
中科院分区：
文献类型：
--
作者：
Date, M;Itaya, H;Kikuchi, Y
目的：检测谷氨酸棒杆菌对人表皮生长因子(HEGF)的分泌。方法与结果：我们最近发现了一种新的谷氨酸链霉菌蛋白分泌系统，可以产生莫巴拉链霉菌转谷氨酰胺酶。在本研究中，谷氨酸杆菌以完全活性的形式将具有工业意义的人表皮生长因子蛋白分泌到培养液中，并以高达156mg·L(-1)天(-1)的速率积累。结论：这些结果表明谷氨酸杆菌能够以活性形式分泌人表皮生长因子蛋白。此外，我们还证明了该细菌有潜力作为工业规模生产人类蛋白质的宿主。
Aims: To examine the secretion of human epidermal growth factor (hEGF) by Corynebacterium glutamicum.Methods and Results: We recently showed that a novel protein-secretion system in C. glutamicum could produce Streptomyces mobaraensis transglutaminase. In the present study, the industrially important protein hEGF was secreted into the culture medium in a fully active form by C. glutamicum and accumulated at a rate of up to 156 mg l(-1) day(-1).Conclusions: These results demonstrated that the hEGF protein could be secreted in an active form by C. glutamicum.Significance and Impact of the Study: Our data confirmed that the pharmaceutically important human protein hEGF could be efficiently secreted in an active form by the C. glutamicum protein-expression system. Moreover, we demonstrated that this bacterium has potential as a host for the industrial-scale production of human proteins.