The stability of human respiratory syncytial virus is enhanced by incorporation of the baculovirus GP64 protein.
The stability of human respiratory syncytial virus is enhanced by incorporation of the baculovirus GP64 protein.
复制标题
杆状病毒 GP64 蛋白的掺入增强了人呼吸道合胞病毒的稳定性。
DOI：
10.1016/j.vaccine.2007.04.066
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发表时间：
2007
期刊：
影响因子：
5.5
通讯作者：
Wertz,GailW
中科院分区：
文献类型：
--
作者：
Sastre,Patricia;Oomens,AntoniusGP;Wertz,GailW
目前研制抗人呼吸道合胞病毒（HRSV）疫苗的努力主要集中在减毒活株上。然而，HRSV的不稳定性是制备、储存和分发候选活疫苗的主要挑战。我们在此报道，加入加州自状杆状病毒多核多角体病毒的GP64糖蛋白可以提高HRSV的稳定性。GP64代替或加入同源HRSV糖蛋白，从HRSV基因组中表达，或通过在组成性表达GP64的Vero细胞系（Vbac细胞）中繁殖病毒来提供。在4°、22°和37°C条件下保存8周，监测不同病毒库的传染性。结果表明，GP64蛋白在22℃保存6周后，HRSV的传染性可稳定10000倍。这种稳定HRSV活病毒的方法可能对疫苗开发很重要，也可能对稳定其他包膜病毒有用。
Current efforts to develop a vaccine against human respiratory syncytial virus (HRSV) are focused on live attenuated strains. However, the unstable nature of HRSV is a major challenge for the preparation, storage and distribution of live vaccine candidates. We report here that the stability of HRSV can be improved by incorporation of the GP64 glycoprotein from baculovirus Autographa californica multiple nucleopolyhedrovirus. GP64 was incorporated in place of or in addition to the homologous HRSV glycoproteins and was either expressed from the HRSV genome or provided by propagating the virus in a Vero cell line constitutively expressing GP64 (Vbac cells). The infectivity of the different virus stocks was monitored after storage at 4°, 22° or 37°C, over a period of 8 weeks. The results showed that the infectivity of HRSV could be stabilized by up to 10,000-fold by the GP64 protein, when stored at 22°C for 6 weeks. This approach for stabilizing live HRSV may be important for vaccine development and may also prove useful for stabilizing other enveloped viruses.