CmWRKY15 Facilitates Alternaria tenuissima Infection of Chrysanthemum.
CmWRKY15 Facilitates Alternaria tenuissima Infection of Chrysanthemum.
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CmWRKY15 促进菊花链格孢感染
DOI：
10.1371/journal.pone.0143349
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Fan Q;Song A;Xin J;Chen S;Jiang J;Wang Y;Li X;Chen F
脱落酸（ABA）能够诱导气孔关闭并与水杨酸（SA）和茉莉酸（JA）相互作用，在植物对病原体的反应中起着重要作用。WRKY转录因子在植物对多种病原体的反应中起拮抗或协同调节作用。在这里，我们证明了CmWRKY15，一个IIa组WRKY家族成员，在酵母细胞中没有转录激活。短暂转染洋葱表皮细胞CmWRKY15的亚细胞定位实验表明，CmWRKY15在体内定位于细胞核。CmWRKY15基因的表达可在菊花中明显诱导。此外，CmWRKY15过表达植株的疾病严重程度指数（DSI）数据表明，与对照相比，CmWRKY15过表达增强了菊花对黄叶菊感染的敏感性。为了阐明CmWRKY15调控黄豆接种应答的机制，我们比较了转基因植株和对照植株中ABA应答基因和ABA信号基因ABF4、ABI4、ABI5、MYB2、RAB18、DREB1A、DREB2A、PYL2、PP2C、RCAR1、SnRK2.2、SnRK2.3、NCED3A、NCED3B、GTG1、AKT1、AKT2、KAT1、KAT2和kc1的表达水平。综上所述，我们的数据表明CmWRKY15可能通过直接或间接拮抗ABA应答基因和ABA信号相关基因的表达来促进蛇麻草的感染。
Abscisic acid (ABA) has an important role in the responses of plants to pathogens due to its ability to induce stomatal closure and interact with salicylic acid (SA) and jasmonic acid (JA). WRKY transcription factors serve as antagonistic or synergistic regulators in the response of plants to a variety of pathogens. Here, we demonstrated that CmWRKY15, a group IIa WRKY family member, was not transcriptionally activated in yeast cells. Subcellular localization experiments in which onion epidermal cells were transiently transfected with CmWRKY15 indicated that CmWRKY15 localized to the nucleus in vivo. The expression of CmWRKY15 could be markedly induced by the presence of Alternaria tenuissima inoculum in chrysanthemum. Furthermore, the disease severity index (DSI) data of CmWRKY15-overexpressing plants indicated that CmWRKY15 overexpression enhanced the susceptibility of chrysanthemum to A. tenuissima infection compared to controls. To illustrate the mechanisms by which CmWRKY15 regulates the response to A. tenuissima inoculation, the expression levels of ABA-responsive and ABA signaling genes, such as ABF4, ABI4, ABI5, MYB2, RAB18, DREB1A, DREB2A, PYL2, PP2C, RCAR1, SnRK2.2, SnRK2.3, NCED3A, NCED3B, GTG1, AKT1, AKT2, KAT1, KAT2, and KC1were compared between transgenic plants and controls. In summary, our data suggest that CmWRKY15 might facilitate A. tenuissima infection by antagonistically regulating the expression of ABA-responsive genes and genes involved in ABA signaling, either directly or indirectly.