Measurement of 2',5'-oligoadenylate synthetase in patients receiving interferon-alpha.
Measurement of 2',5'-oligoadenylate synthetase in patients receiving interferon-alpha.
复制标题
接受干扰素-α 治疗的患者中 2,5-寡腺苷酸合成酶的测量。
DOI：
10.1089/jir.1985.5.191
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Ball,LA
中科院分区：
文献类型：
--
作者：
Merritt,JA;Borden,EC;Ball,LA
在28名正常健康人和14名接受干扰素- α注射的患者的ficoll纯化的人外周血单个核细胞提取物中，测定了干扰素诱导的细胞内酶2 ',5 ' -寡腺苷酸（2,5a）合成酶。在2 × 106个细胞中可重复测量基础水平和刺激水平。在两组中，单个核细胞的2,5a合成酶水平变化很大，但在每个个体中测量是可重复的。在大多数情况下，在干扰素- α给药后8小时内检测到酶活性增加。升高的水平持续了至少24小时，并通过每日治疗得以维持。在14名患者中，有两名患者多次注射干扰素后酶水平没有反应。有趣的是，这两名患者的预处理酶水平明显高于健康人群。测量2,5a合成酶水平对临床干扰素研究是一个有价值的补充，因为结果可能有助于解决干扰素给药的最佳剂量、途径和计划的问题。
The interferon-induced intracellular enzyme, 2′,5′-oligoadenylate (2,5A) synthetase, was measured in extracts of Ficoll-purified human peripheral mononuclear cells from 28 normal healthy individuals and 14 patients receiving injections of interferon-alpha. Basal and stimulated levels could be measured reproducibly in 2 × 106cells. In both groups, mononuclear cell levels of 2,5A synthetase varied widely, but measurements were reproducible in each individual. In most instances, increases in enzyme activity were detected within 8 h of interferon-alpha administration. Elevated levels persisted for at least 24 h and were maintained with daily treatment. In two of the 14 patients, the enzyme level failed to respond to multiple interferon injections. Interestingly, these two patients had pretreatment enzyme levels that were markedly elevated relative to those of a healthy population. Measurement of 2,5A synthetase levels is a valuable addition to clinical interferon studies, since the results may help to resolve questions of the optimal dose, route, and schedule of interferon administration.