Methoprene-tolerant (Met) and Krüpple-homologue 1 (Kr-h1) are required for ovariole development and egg maturation in the brown plant hopper.
Methoprene-tolerant (Met) and Krüpple-homologue 1 (Kr-h1) are required for ovariole development and egg maturation in the brown plant hopper.
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褐飞虱卵巢发育和卵成熟需要耐甲氧普林 (Met) 和 Krupple 同源物 1 (Kr-h1)
DOI：
10.1038/srep18064
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发表时间：
2015-12-14
影响因子：
4.6
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Lin X;Yao Y;Wang B
褐飞虱是已知的最具破坏性的水稻害虫之一。我们研究了JH受体Met和下游转录因子Kr-h1在卵巢管发育和卵子成熟中的作用。预测的N.褐藻胶（NlMet）含有517个氨基酸。qRT-PCR结果显示NlMet在所有组织中均有表达，且在胚胎期表达量最高。在NlMet或NlKr-h1沉默的雌性成年动物中，卵巢发育变化显著，而在注射靶向NlMet、NlKrh-1或NlMet和NlKr-h1两者的dsRNA的那些中，卵巢管的数量变化较小。在注射dsNlKr-h1或dsNlMet与dsNlKr-h1 dsRNA的组合的雌性中，产卵前期延长，而注射NlMet dsRNA的雌性没有显示出显著变化。此外，我们没有发现长翅型和短翅型雌性之间的产卵前期长度的差异。Nlmet或NlKr-h1的破坏或NlMet和NlKr-h1的双重敲低显著降低了产卵的数量。此外，大翅型和短翅型褐飞虱之间也存在显著差异。这些结果表明，Met和Kr-h1是褐飞虱卵巢管发育和卵成熟所必需的。
The brown plant hopper is one of the most destructive known pests of rice. We studied the roles of the JH receptor Met and the downstream transcription factor Kr-h1 in ovariole development and egg maturation. The predicted Met protein in N. lugens (NlMet) contained 517 amino acids. qRT-PCR showed that NlMet was expressed in all tissues and that the highest expression occurred in the embryonic stage. In NlMet- or NlKr-h1-silenced female adults, ovarian development varied significantly, whereas the numbers of ovarioles were less variable in those injected with dsRNA targeting NlMet, NlKrh-1 or both NlMet and NlKr-h1. In females injected with dsNlKr-h1 or with dsNlMet in combination with dsNlKr-h1 dsRNA, the preoviposition period was prolonged, whereas the females injected with NlMet dsRNA showed no significant changes. Moreover, we found no differences in the length of the preoviposition period between macropterous and brachypterous females. The disruption of Nlmet or NlKr-h1 or the dual knockdown of NlMet and NlKr-h1 significantly reduced the number of eggs laid. Moreover, significant differences were also found between the macropterous and the brachypterous brown plant hoppers. These results indicated that Met and Kr-h1 are required for ovariole development and egg maturation in the brown plant hopper.