Multilayered Ordered Protein Arrays Self-Assembled from a Mixed Population of Virus-like Particles
Multilayered Ordered Protein Arrays Self-Assembled from a Mixed Population of Virus-like Particles
复制标题
由病毒样颗粒混合群自组装的多层有序蛋白质阵列
DOI：
10.1021/acsnano.1c11272
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发表时间：
2022
期刊：
影响因子：
17.1
通讯作者：
Douglas, Trevor
中科院分区：
文献类型：
--
作者：
Uchida, Masaki;Brunk, Nicholas E.;Hewagama, Nathasha D.;Lee, Byeongdu;Prevelige, Peter E.;Jadhao, Vikram;Douglas, Trevor
生物学显示了许多空间控制的细胞和生物大分子组装成分层组织结构的例子，许多复杂的生物功能都归因于此。虽然这种生物结构激发了合成材料的设计，但在将多种类型的组件组装成散装材料时，控制单个构建块的空间排列仍然是一个巨大的挑战。在这里，我们报告自组装的多层，有序的蛋白质阵列从混合人口的病毒样颗粒（VLP）。我们通过诱变系统地调节VLP表面电荷的大小，以制备四种不同类型的VLP用于混合。多达四种类型的VLP的混合物选择性地组装成更高阶的结构，在带相反电荷的树枝状聚合物的存在下，在溶液的离子强度逐渐降低。该组装导致形成三维有序的VLP阵列，其具有多达四个不同的层，包括中心芯，每个层包括单一类型的VLP。一个粗粒度的计算模型的开发和模拟使用分子动力学探测的多层，核壳结构的形成。我们的研究结果建立了一个简单而通用的自下而上的策略，通过控制多种类型的纳米级构建块在一锅制造的空间排列来合成多层，有序的材料。
Biology shows many examples of spatially controlled assembly of cells and biomacromolecules into hierarchically organized structures, to which many of the complex biological functions are attributed. While such biological structures have inspired the design of synthetic materials, it is still a great challenge to control the spatial arrangement of individual building blocks when assembling multiple types of components into bulk materials. Here, we report self-assembly of multilayered, ordered protein arrays from mixed populations of virus-like particles (VLPs). We systematically tuned the magnitude of the surface charge of the VLPs via mutagenesis to prepare four different types of VLPs for mixing. A mixture of up to four types of VLPs selectively assembled into higher-order structures in the presence of oppositely charged dendrimers during a gradual lowering of the ionic strength of the solution. The assembly resulted in the formation of three-dimensional ordered VLP arrays with up to four distinct layers including a central core, with each layer comprising a single type of VLP. A coarse-grained computational model was developed and simulated using molecular dynamics to probe the formation of the multilayered, core-shell structure. Our findings establish a simple and versatile bottom-up strategy to synthesize multilayered, ordered materials by controlling the spatial arrangement of multiple types of nanoscale building blocks in a one-pot fabrication.