Detection of recombinant haplotypes in wild mice (Mus musculus) provides new insights into the origin of Japanese mice
Detection of recombinant haplotypes in wild mice (Mus musculus) provides new insights into the origin of Japanese mice
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DOI：
10.1111/j.1365-294x.2010.04651.x
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发表时间：
2010-06-01
影响因子：
4.9
通讯作者：
Suzuki, Hitoshi
中科院分区：
文献类型：
--
作者：
Nunome, Mitsuo;Ishimori, Chikako;Suzuki, Hitoshi
日本家鼠（Mus musculus molossinus）被认为是源自两种史前移民的杂交谱系，即 M. m. 亚种。欧亚大陆北部的肌肉和 M. m.南亚的栗。西欧亚种小鼠 M. m.仅在日本港口和机场检测到了domesticus。我们检查了来自欧亚大陆 59 只小鼠 8 号染色体上 200 kb 片段的单倍型结构，确定了按此顺序间歇排列的 8 个核基因（Fanca、Spire2、Tcf25、Mc1r、Tubb3、Def8、Afg3l1 和 Dbndd1）的短片段（大约 370-600 bp）。在可能的情况下，我们确定了单个基因等位基因的亚种起源，然后指定了串联等位基因的单倍型。我们在检查的 19 只日本小鼠中恢复了 11 种单倍型，被鉴定为源自肌肉亚种 (57.9%)、家鼠亚种 (7.9%) 和栗亚种 (2.6%) 的“完整”单倍型，或被鉴定为“重组”单倍型 (31.6%)。我们还检测到了萨哈林岛特有的重组单倍型。重组单倍型的复杂性质表明，所有三个亚种成分在从源区迁移之前就被古代引入了欧亚大陆的外围部分或复杂的基因组混合物。其他日本野生小鼠中“完整”的家鼠和栗单倍型意味着持续的偷渡引入。该方法对于评估基因混合的历史和披露正在进行的基因污染具有普遍实用性。
Japanese house mice (Mus musculus molossinus) are thought to be a hybrid lineage derived from two prehistoric immigrants, the subspecies M. m. musculus of northern Eurasia and M. m. castaneus of South Asia. Mice of the western European subspecies M. m. domesticus have been detected in Japanese ports and airports only. We examined haplotype structuring of a 200 kb stretch on chromosome 8 for 59 mice from throughout Eurasia, determining short segments (approximate to 370-600 bp) of eight nuclear genes (Fanca, Spire2, Tcf25, Mc1r, Tubb3, Def8, Afg3l1 and Dbndd1) which are intermittently arranged in this order. Where possible we identified the subspecies origin for individual gene alleles and then designated haplotypes for concatenated alleles. We recovered 11 haplotypes among 19 Japanese mice examined, identified either as 'intact' haplotypes derived from the subspecies musculus (57.9%), domesticus (7.9%), and castaneus (2.6%), or as 'recombinant' haplotypes (31.6%). We also detected recombinant haplotypes unique to Sakhalin. The complex nature of the recombinant haplotypes suggests ancient introduction of all three subspecies components into the peripheral part of Eurasia or complicated genomic admixture before the movement from source areas. 'Intact' domesticus and castaneus haplotypes in other Japanese wild mice imply ongoing stowaway introductions. The method has general utility for assessing the history of genetic admixture and for disclosing ongoing genetic contamination.