Ataxia telangiectasia mutated deficiency does not result in genetic susceptibility to 50Hz magnetic fields exposure in mouse embryonic fibroblasts
Ataxia telangiectasia mutated deficiency does not result in genetic susceptibility to 50Hz magnetic fields exposure in mouse embryonic fibroblasts
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共济失调毛细血管扩张突变缺陷不会导致小鼠胚胎成纤维细胞对暴露于 50 Hz 磁场的遗传易感性。
DOI：
10.1002/bem.22140
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发表时间：
2018-09-01
影响因子：
1.9
通讯作者：
Chen, Guangdi
中科院分区：
文献类型：
--
作者：
Sun, Chuan;Wei, Xiaoxia;Chen, Guangdi
极低频磁场（ELF-MF）已被国际癌症研究机构（International Agency for Research on Cancer）列为可能对人类致癌的物质[2002]。然而，由于缺乏足够的实验证据，关于ELF-MF基因毒性效应的争论仍在继续。共济失调毛细血管扩张突变（ATM）在DNA损伤修复中起核心作用；它的缺乏会导致细胞对dna损伤剂敏感。为了评估ELF-MF的遗传毒性，我们研究了50Hz MF对Atm -精通（Atm(+/+)）小鼠胚胎成纤维细胞（mef）和Atm -缺乏（Atm(-/-)） mef（一种辐射敏感细胞系）DNA损伤的影响。结果显示，当Atm(+/+) MEFs在2.0mT 50Hz MF下暴露15min时，假手术组和暴露组之间每个细胞平均H2AX灶数（9.37 +/- 0.44 vs 9.08 +/- 0.28， P=0.58）或H2AX灶阳性细胞百分比（49.22 +/- 1.86% vs 49.74 +/- 1.44%， P=0.83）无显著差异。延长暴露时间至1或24小时对Atm(+/+) mef中H2AX灶的形成没有显著影响。同样，暴露对Atm(-/-) mef中H2AX灶的形成也没有显著影响。此外，50Hz MF暴露对两种细胞类型的DNA断裂、细胞活力或细胞周期进展也没有显著影响。综上所述，在报道的实验条件下，暴露于50Hz MF均未引起Atm（+/+）或Atm(-/-) mef的显著DNA损伤。中国生物医学工程学报，2018,39(1):476-484。(c) 2018 Wiley期刊公司
Extremely low frequency magnetic field (ELF-MF) has been classified as a possible carcinogen to humans by the International Agency for Research on Cancer [2002]. However, debate on the genotoxic effects of ELF-MF has continued due to lack of sufficient experimental evidence. Ataxia telangiectasia mutated (ATM) plays a central role in DNA damage repair; its deficiency can result in cellular sensitivity to DNA-damaging agents. To evaluate the genotoxicity of ELF-MF, we investigated the effects of 50Hz MF on DNA damage in ATM-proficient (Atm(+/+)) mouse embryonic fibroblasts (MEFs) and ATM-deficient (Atm(-/-)) MEFs, a radiosensitive cell line. Results showed no significant difference in average number of H2AX foci per cell (9.37 +/- 0.44 vs. 9.08 +/- 0.28, P=0.58) or percentage of H2AX foci positive cells (49.22 +/- 1.86% vs. 49.74 +/- 1.44%, P=0.83) between sham and exposure groups when Atm(+/+) MEFs were exposed to 50Hz MF at 2.0mT for 15min. Extending exposure duration to 1 or 24h did not significantly change H2AX foci formation in Atm(+/+) MEFs. Similarly, the exposure did not significantly affect H2AX foci formation in Atm(-/-) MEFs. Furthermore, 50Hz MF exposure also did not significantly influence DNA fragmentation, cell viability, or cell cycle progression in either cell types. In conclusion, exposure to 50Hz MF did not induce significant DNA damage in either Atm(+/+) or Atm(-/-) MEFs under the reported experimental conditions. Bioelectromagnetics. 39:476-484, 2018. (c) 2018 Wiley Periodicals, Inc.