The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly
The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly
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DOI：
10.1091/mbc.e06-02-0133
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发表时间：
2006-09-01
影响因子：
3.3
通讯作者：
Pazour, Gregory J.
中科院分区：
文献类型：
--
作者：
Follit, John A.;Tuft, Richard A.;Pazour, Gregory J.
真核细胞纤毛通过鞭毛内转运（IFT）组装，其中大的蛋白质颗粒沿沿着纤毛微管运动。IFT颗粒由至少17种多肽组成，这些多肽被认为含有各种货物的结合位点，这些货物需要从细胞体中的合成位点转运到纤毛中的组装位点。我们在这里表明，粒子的IFT 20亚基是本地化的高尔基复合体除了基体和纤毛，在所有以前的IFT粒子proteins. In活细胞中，荧光标记的IFT 20是高度动态和高尔基复合体和纤毛之间的移动以及沿着纤毛微管。在哺乳动物细胞中，强烈敲低的IFFT 20阻断纤毛组装，但不影响高尔基体结构。中度敲除不会阻止纤毛组装，但会减少定位于纤毛的多囊蛋白-2的量。这项工作表明，IFFT 20的功能，从高尔基复合体的纤毛膜蛋白的交付纤毛。
Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT particles are composed of at least 17 polypeptides that are thought to contain binding sites for various cargos that need to be transported from their site of synthesis in the cell body to the site of assembly in the cilium. We show here that the IFT20 subunit of the particle is localized to the Golgi complex in addition to the basal body and cilia where all previous IFT particle proteins had been found. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. Strong knock down of IFT20 in mammalian cells blocks ciliary assembly but does not affect Golgi structure. Moderate knockdown does not block cilia assembly but reduces the amount of polycystin-2 that is localized to the cilia. This work suggests that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium.