Influence of dietary fibers on fermentation in the human large intestine.
Influence of dietary fibers on fermentation in the human large intestine.
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膳食纤维对人体大肠发酵的影响。
DOI：
10.1093/jn/112.1.158
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
P. V. Van Soest
中科院分区：
文献类型：
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作者：
F. R. Ehle;J. Robertson;P. V. Van Soest
给 24 名男性喂食纤维源，以评估纤维对大肠微生物群的影响。日粮为低纤维基础日粮或补充有粗麸皮、细麸皮、卷心菜或纤维素（Solka floc）的基础日粮。 12 名受试者（第 1 组）使用拉丁方设计喂食所有纤维来源，实验期为 2 周，随后为基础饮食 3 周。第 2 组在第一个实验期后改变了饮食习惯，然后在整个实验过程中保持这种饮食习惯；在最后一个时期，他们的纤维摄入量增加了一倍（达到约 26 克/天）。利用微生物培养系统和粪便微生物群来测量细胞壁难消化性和挥发性脂肪酸（VFA）的产生。还测量了新鲜粪便中的挥发性脂肪酸。体外基质是苜蓿、粗麸、卷心菜或纤维素。在该系统中，膳食纤维对最终的细胞壁难消化性和 VFA 水平具有显着影响（P 小于 0.05）。第 1 组受试者先前的饮食显着（P 小于 0.05）影响体外 VFA。在第 2 组中，体外 VFA 数据存在显着（P 小于 0.05）的周期和受试者效应。人类粪便中的 VFA 浓度受到消耗的纤维的显着影响。
Fiber sources were fed to 24 men to evaluate the effect of fiber on large intestinal microflora. The diets were low fiber basal or the basal diet supplemented with coarse bran, fine bran, cabbage, or cellulose (Solka floc). Twelve subjects (Group 1) were fed all fiber sources using a latin square design with 2-week experimental periods followed by a 3-week period on the basal diet. Group 2 made one diet change after the first experimental period, then remained on that diet throughout the experiment; during the last period their fiber intake was double (to approximately 26 g/day). A microbial culture system was utilized with fecal microflora to measure cell wall indigestibility and volatile fatty acid (VFA) production. Volatile fatty acids were also measured in fresh feces. The in vitro substrates were alfalfa, coarse bran, cabbage, or cellulose. In this system, dietary fiber had a significant (P less than .05) effect on resultant cell wall indigestibility and VFA level. Previous diet in group 1 subjects significantly (P less than .05) affected in vitro VFA. In groups 2 significant (P less than .05) period and subject effect were present for the in vitro VFA data. The VFA concentrations in feces of humans were significantly affected by the fiber consumed.