Transcriptional Analysis of Host Responses to Marek's Disease Virus Infection in Chicken Thymus
Transcriptional Analysis of Host Responses to Marek's Disease Virus Infection in Chicken Thymus
复制标题
鸡胸腺中马立克氏病病毒感染宿主反应的转录分析
DOI：
10.1159/000370069
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发表时间：
2015-01-01
期刊：
影响因子：
4.6
通讯作者：
Ye, Jianqiang
中科院分区：
文献类型：
--
作者：
Hu, Xuming;Qin, Aijian;Ye, Jianqiang
马立克氏病病毒（MDV）是一种细胞相关的α -疱疹病毒，可引起鸡的t细胞淋巴瘤和神经紊乱。与其他淋巴器官不同，胸腺是t细胞成熟和分化的场所。然而，鸡胸腺对MDV感染的转录反应尚不清楚。在本研究中，我们对感染rb1b的鸡胸腺组织在不同时间点的全基因组表达进行了分析，以探讨MDV发病的分子机制。2倍及以上变化的差异表达基因（bbb2）数量为：1250个基因（7 dpi）， 834个基因（14 dpi）， 1958个基因（21 dpi）， 2306个基因（28 dpi）。基因本体富集分析显示，上调基因在7 dpi时参与免疫和炎症反应；血管生成、细胞骨架组织、细胞粘附和信号转导在21和28 dpi时表现出不同的表达。随机选取18个基因，用实时荧光定量pcr法确定其表达谱。讨论了几种与肿瘤发展相关的不同表达的宿主基因。我们确定了对MDV感染有反应的鸡胸腺中宿主基因的全球表达模式。本研究结果可为进一步研究MDV的生物学和发病机制奠定基础。
Marek's disease virus (MDV) is a cell-associated alpha-herpesvirus that causes T-cell lymphomas and nervous disorders in chickens. Different from other lymphoid organs, the thymus is the site of T-cell maturation and differentiation. However, the transcriptional response to MDV infection in the chicken thymus is still not known. In this study, we performed genome-wide expression analysis in thymus tissues of RB1B-infected chickens at different time points to investigate the molecular mechanisms of MDV pathogenesis. The number of differentially expressed genes with 2-fold or higher changes (>2) are as follows: 1,250 genes (7 dpi), 834 genes (14 dpi), 1,958 genes (21 dpi), and 2,306 genes (28 dpi). Gene ontology enrichment analysis revealed that the upregulated genes were involved in immune and inflammatory response at 7 dpi; angiogenesis, cytoskeleton organization, cell adhesion, and signal transduction showed different expressions at 21 and 28 dpi. The expression pattern of 18 randomly selected genes was confirmed by real-time RT-PCR. Several differently expressed host genes associated with tumor development are discussed. We identified the global host-gene expression pattern in the thymus of chickens that responded to MDV infection. The present data may provide groundwork for future investigation in the biology and pathogenesis of MDV.