Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
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DOI：
10.1038/bjc.1983.56
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发表时间：
1983-03
影响因子：
8.8
通讯作者：
Brattain, D E
中科院分区：
文献类型：
--
作者：
Brattain, M G;Marks, M E;McCombs, J;Finely, W;Brattain, D E
描述了在汇合成纤维细胞的饲养层上培养的人结肠癌细胞系的起始。尝试在没有成纤维细胞饲养层的情况下启动培养没有成功。从饲养层表面生长的细胞中分离并断奶两个亚系（命名为 HCT C 和 HCT C Col）。这些亚系具有不同的形态，将不同水平的癌胚抗原（CEA）分泌到汇合培养物的培养基中，并且具有稍微不同的核型。两个亚系均在半固体培养基中生长，并在皮下注射时形成异种移植物。进入无胸腺裸鼠。放射性碘标记的细胞膜成分分析表明亚系之间存在微小但显着的差异。
The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines.