Evaluating the effects of genetic variants of DNA repair genes using cytogenetic mutagen sensitivity approaches.
Evaluating the effects of genetic variants of DNA repair genes using cytogenetic mutagen sensitivity approaches.
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DOI：
10.3109/1354750x.2011.577237
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发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
El-Zein RA
中科院分区：
文献类型：
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作者：
Abdel-Rahman SZ;El-Zein RA
通过细胞遗传学终点在外周血淋巴细胞的短期培养物中测量的诱变剂敏感性是DNA修复能力的间接测量，多年来一直用作癌症内在易感性的生物标志物。在这篇文章中，我们简要地概述了不同的细胞遗传学诱变剂的敏感性的方法，已成功地用于评估DNA修复基因多态性的生物学效应的基础上，目前的文献综述和生物标志物的需要，这将允许这种多态性的生物学和功能意义的表征。我们还讨论了这一新兴研究领域面临的一些未来挑战。
Mutagen sensitivity, measured in short-term cultures of peripheral blood lymphocytes by cytogenetic endpoints, is an indirect measure for DNA repair capacity and has been used for many years as a biomarker for intrinsic susceptibility for cancer. In this article, we briefly give an overview of the different cytogenetic mutagen sensitivity approaches that have been used successfully to evaluate the biological effects of polymorphisms in DNA repair genes based on a current review of the literature and based on the need for biomarkers that would allow the characterization of the biological and functional significance of such polymorphisms. We also address some of the future challenges facing this emerging area of research.