CLONING OF A GENE FROM PSEUDOMONAS SP STRAIN PG2982 CONFERRING INCREASED GLYPHOSATE RESISTANCE
CLONING OF A GENE FROM PSEUDOMONAS SP STRAIN PG2982 CONFERRING INCREASED GLYPHOSATE RESISTANCE
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DOI：
10.1128/aem.56.11.3382-3388.1990
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发表时间：
1990-11-01
影响因子：
4.4
通讯作者：
BRAYMER, HD
中科院分区：
文献类型：
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作者：
FITZGIBBON, JE;BRAYMER, HD
从假单胞菌菌株PG 2982中分离出一个携带2.4-磷酸酶对DNA片段的质粒，该质粒能够增加大肠杆菌细胞的草甘膦抗性。抗性的增加依赖于分子量约为33，000的质粒编码蛋白的存在，该蛋白是载体pACYC 184上的基因与插入DNA之间的翻译融合产物。克隆了携带整个基因（命名为igrA）的PG 2982染色体的重叠区域，并且携带该基因的质粒（pPG 18）也能够增加E.杆菌pPG 18中所含的PG 2982 DNA编码分子量约为40，000的蛋白质。该质粒不能互补E.大肠杆菌（E. coli）对草甘膦进行修饰。不能证明含有质粒的大肠杆菌细胞。PG 2982 DNA的核苷酸序列含有开放阅读框，能够编码计算分子量为39，396的蛋白质。
A plasmid carrying a 2.4-kilobase-pair fragment of DNA from Pseudomonas sp. strain PG2982 has been isolated which was able to increase the glyphosate resistance of Escherichia coli cells. The increase in resistance was dependent on the presence of a plasmid-encoded protein with a molecular weight of approximately 33,000, the product of a translational fusion between a gene on the vector, pACYC184, and the insert DNA. An overlapping region of the PG2982 chromosome carrying the entire gene (designated igrA) was cloned, and a plasmid (pPG18) carrying the gene was also able to increase glyphosate resistance in E. coli. A proteion with a molecular weight of approximately 40,000 was encoded by the PG2982 DNA contained in pPG18. This plasmid was not able to complement a mutation in the gene for 5-enolpyruvylshikimate-3-phosphate synthase (aroA) in E. coli, and modification of glyphosate by E. coli cells containing the plasmid could not be demonstrated. The nucleotide sequence of the PG2982 DNA contained an open reading frame able to encode a protein with a calculated molecular weight of 39,396.