Proteomic Analysis of the Royal Jelly and Characterization of the Functions of its Derivation Glands in the Honeybee
Proteomic Analysis of the Royal Jelly and Characterization of the Functions of its Derivation Glands in the Honeybee
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DOI：
10.1021/pr300700e
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发表时间：
2013-01-01
影响因子：
4.4
通讯作者：
Kubo, Takeo
中科院分区：
文献类型：
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作者：
Fujita, Toshiyuki;Kozuka-Hata, Hiroko;Kubo, Takeo
为了鉴定可能影响蜂群成员生理状态的候选蜂王浆（RJ）蛋白，我们采用散弹枪蛋白质组学方法对RJ蛋白组以及RJ蛋白来源于的下咽腺（HpG）、脑后腺（PcG）和胸腺（TG）的蛋白质组进行了综合鉴定。共鉴定出38个非冗余RJ蛋白，包括22个推定分泌蛋白和胰岛素样生长因子结合蛋白复合物酸不稳定亚基。其中9个蛋白为新鉴定的RJ蛋白。将RJ蛋白质组与HpG、PcG和TG蛋白质组进行比较，发现22个推定分泌的RJ蛋白质中有17个来自这些腺体，这表明RJ蛋白质组是来自这三个腺体的蛋白质的混合物。此外，途径分析表明，HpG蛋白质组代表了极高蛋白质合成能力的分子基础，而PcG蛋白质组表明PcG作为挥发性化合物的储存库和引物信息素。最后，为了进一步表征可能的RJ总蛋白质组，我们在这三个腺体的蛋白质组中鉴定了假定的分泌蛋白。这将有助于在今后的研究中预测新的RJ蛋白成分。
To identify candidate royal jelly (RJ) proteins that might affect the physiologic status of honeybee colony members, we used shotgun proteomics to comprehensively identify the RJ proteome as well as proteomes of the hypopharyngeal gland (HpG), postcerebral gland (PcG), and thoracic gland (TG), from which RJ proteins are assumed to be derived. We identified a total of 38 nonredundant RJ proteins, including 22 putative secretory proteins and Insulinlike growth factor-binding protein complex acid labile subunit. Among them, 9 proteins were newly identified from RJ. Comparison of the RJ proteome with the HpG, PcG, and TG proteomes revealed that 17 of the 22 putative secretory RJ proteins were derived from some of these glands, suggesting that the RJ proteome is a cocktail of proteins from these three glands. Furthermore, pathway analysis suggested that the HpG proteome represents the molecular basis of the extremely high protein-synthesizing ability, whereas the PcG proteome suggests that the PcG functions as a reservoir for the volatile compounds and a primer pheromone. Finally, to further characterize the possible total RJ proteome, we identified putative secretory proteins in the proteomes of these three glands. This will be useful for predicting novel RJ protein components in future studies.