THE EFFECT OF OXYTOCIN ON PROGESTERONE SECRETION AND OF PGF2-ALPHA ON OXYTOCIN SECRETION FROM BOVINE LUTEAL AND GRANULOSA-CELLS IN CULTURE
THE EFFECT OF OXYTOCIN ON PROGESTERONE SECRETION AND OF PGF2-ALPHA ON OXYTOCIN SECRETION FROM BOVINE LUTEAL AND GRANULOSA-CELLS IN CULTURE
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DOI：
10.1016/0378-4320(90)90022-8
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发表时间：
1990-07-01
影响因子：
2.2
通讯作者：
WATHES, DC
中科院分区：
文献类型：
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作者：
BARRETT, J;WATHES, DC
催产素对hCG刺激的体外培养12 h的牛黄体细胞孕酮分泌有不同的影响。在八个实验中的两个中，高剂量的催产素（>10-9 M）抑制孕酮的分泌，而在另外四个实验中，孕酮的释放被催产素在10-10至10-6 M的浓度范围内刺激。当使用黄体化颗粒细胞时，催产素（10-9至10-6 M）倾向于刺激孕酮输出，但在少数情况下也观察到抑制。在培养的前3小时，黄体中期细胞的催产素释放迅速下降，在12小时内，细胞几乎释放了全部的催产素。然而，在一些实验中，120小时后仍可以在培养基中测量到低浓度的催产素。在培养过程中释放的催产素的量超过了在每个实验开始时黄体细胞中存在的浓度，这表明在体外发生了有限量的合成和/或加工。催产素的释放模式不受血清或hCG在培养基中的存在。PGF 2 α的加入。在长期培养期间，三个实验中有两个减少了催产素的输出。
Oxytocin had a variable effect on progesterone output from hCG-stimulated bovine luteal cells cultured for 12 h in vitro. In two out of eight experiments high doses of oxytocin (>10-9 M) inhibited progesterone output, whereas in a further four experiments progesterone release was stimulated by oxytocin at a range of concentrations from 10-10 to 10-6 M. When luteinized granulosa cells were used, oxytocin (10-9 to 10-6 M) tended to stimulate progesterone output, but in a few cases inhibition was also observed. Oxytocin release from isolated mid-cycle luteal cells declined rapidly during the first 3 h in culture, and the cells had released nearly their entire store of oxytocin within 12 h. Nevertheless, in some experiments, low concentrations of oxytocin could still be measured in the medium after 120 h. The amount of oxytocin released during culture exceeded the concentration present in the luteal cells at the start of each experiment, suggesting that a limited amount of synthesis and/or processing was occurring in vitro. The release pattern of oxytocin was not influenced by the presence of serum or hCG in the medium. The addition of PGF2.alpha. during long-term cultures decreased oxytocin output in two out of three experiments.