Nitroproteins from a human pituitary adenoma tissue discovered with a nitrotyrosine affinity column and tandem mass spectrometry
Nitroproteins from a human pituitary adenoma tissue discovered with a nitrotyrosine affinity column and tandem mass spectrometry
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DOI：
10.1016/j.ab.2006.05.024
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发表时间：
2006-07-15
影响因子：
2.9
通讯作者：
Desiderio, Dominic M.
中科院分区：
文献类型：
--
作者：
Zhan, Xianquan;Desiderio, Dominic M.
本研究的目的是表征内源性氮蛋白。以及与硝基蛋白相互作用的蛋白质，以阐明蛋白质硝化在腺瘤中的作用。一个硝基酪氨酸亲和柱（NTAC）被用来优先富集和分离内源性硝基蛋白和硝基蛋白-蛋白复合物从组织匀浆，从人垂体无功能垂体腺瘤制备。优先富集的内源性氮蛋白和氮蛋白-蛋白复合物进行胰蛋白酶消化。海水淡化和串联质谱分析。9种硝基蛋白（Rho-GT3-活化蛋白5、白细胞免疫球蛋白样受体亚家族A成员4前体、锌指蛋白432、cAMP依赖性蛋白激酶I型β调节亚基、鞘氨醇-1-磷酸裂解酶1。半人马座β 1。蛋白酶体亚单位α 2型、白细胞介素I家族成员6和rhophilin 2）和三种与硝基蛋白相互作用的蛋白质（白细胞介素I受体相关激酶样2、谷氨酸受体相互作用蛋白2和泛素）。每个硝基蛋白的硝化位点都位于发生硝化的功能域上。每一个含氮蛋白都与相应的功能系统有关。这些数据表明，蛋白质硝化可能是一个重要的分子事件，在人类垂体无功能腺瘤的形成。(c)2006年爱思唯尔公司All rights reserved.
The aim of this Study was to characterize endogenous nitroproteins. and those proteins that interact with nitroproteins, in a human pituitary nonfunctional adenoma so as to clarify the role of protein nitration in adenomas. A nitrotyrosine affinity column (NTAC) was used to preferentially enrich and isolate endogenous nitroproteins and nitroprotein-protein complexes from a tissue homogenate that was prepared from a human pituitary nonfunctional pituitary adenoma. The preferentially enriched endogenous nitroproteins and nitroprotein-protein complexes were subjected to trypsin digestion. desalination. and tandem mass spectrometry analysis. Nine nitroproteins (Rho-GTPase-activing protein 5, leukocyte immunoglobulin-like receptor subfamily A member 4 precursor, zinc finger protein 432, cAMP-dependent protein kinase type I-beta regulatory subunit, sphingosine-1-phosphate lyase 1. centaurin beta 1. proteasome subunit alpha type 2, interleukin I family member 6, and rhophilin 2) and three proteins (interleukin I receptor-associated kinase-like 2, glutamate receptor-interacting protein 2, and ubiquitin) that interacted with nitroproteins were discovered. The nitration site ofeach nitroprotein was located onto the functional domain where nitration occurred. and each nitroprotein was related to a corresponding functional system. Those data indicate that protein nitration might be an important molecular event in the formation of a human pituitary nonfunctional adenoma. (c) 2006 Elsevier Inc. All rights reserved.