INVITRO AND INVIVO UPTAKE OF BENZOPORPHYRIN DERIVATIVE INTO HUMAN AND MINISWINE ATHEROSCLEROTIC PLAQUE
INVITRO AND INVIVO UPTAKE OF BENZOPORPHYRIN DERIVATIVE INTO HUMAN AND MINISWINE ATHEROSCLEROTIC PLAQUE
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DOI：
10.1111/j.1751-1097.1993.tb02935.x
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发表时间：
1993-04-01
影响因子：
3.3
通讯作者：
LEVY, JG
中科院分区：
文献类型：
--
作者：
HSIANG, YN;CRESPO, MT;LEVY, JG
苯并卟啉衍生物（BPD）是一种新型的光动力学治疗光敏剂。虽然大多数关于BPD的工作都集中在其在癌症治疗中的潜在应用上，但BPD在动脉粥样硬化治疗中可能具有潜在的临床用途。本研究的目的是确定在体外和体内摄取的BPD进入动脉粥样硬化斑块。将动脉粥样硬化的人股动脉和腘动脉样品与以下浓度的BPD-一元酸A环（BPD-MA）孵育1小时：1、5、10、20、30和40 mu/mL。通过用分光荧光计进行化学提取来测定来自所有样品的荧光。人体动脉的组织浓度分别为0.37 +/- 0.03、2.78 +/- 1.5、3.6 +/- 1.91、7.15 +/- 2.36、8.06 +/- 3.09和14.6 +/- 4.81 mug/g。此外，三只小型猪动脉粥样硬化，静脉注射BPD 2.0 mg/kg。BPD-MA在小型猪主动脉中的浓度为93-190 ng/g，斑块/正常比值为1.7-3.5。小型猪髂动脉[BPD-MA]为60-178 ng/g，斑块/正常值为1.1-3.3。本研究表明，BPD-MA在体内外动脉粥样硬化血管中均被摄取，可能具有动脉粥样硬化PDT的潜力。
Benzoporphyrin derivative (BPD) has been demonstrated to be a new potent photosensitizer for photodynamic therapy (PDT). Although most of the work on BPD has been focused on its potential applications for cancer treatment, BPD may have potential clinical uses in the treatment of atherosclerosis. The purpose of this study was to determine in vitro and in vivo uptake of BPD into atherosclerotic plaque. Samples of atherosclerotic human femoral and popliteal arteries were incubated with BPD-monoacid, ring A (BPD-MA) for 1 h in the following concentrations: 1, 5, 10, 20, 30 and 40 mu/mL. Fluorescence from all samples was determined by chemical extraction with a spectrofluorometer. The tissue concentration for human arteries was 0.37 +/- 0.03, 2.78 +/- 1.5, 3.6 +/- 1.91, 7.15 +/- 2.36, 8.06 +/- 3.09 and 14.6 +/- 4.81 mug/g, respectively. In addition, three miniswine were rendered atherosclerotic and given BPD 2.0 mg/kg intravenously. The concentration of BPD-MA in miniswine aorta was 93-190 ng/g and the plaque/normal ratio was 1.7-3.5. For miniswine iliac arteries, the [BPD-MA] was 60-178 ng/g and the plaque/normal ratio was 1.1-3.3 Normal miniswine carotid artery contained 54 ng/g. This study showed that BPD-MA was taken up in atherosclerotic vessels both in vitro and in vivo and may have potential for PDT of atherosclerosis.