Charge-charge interactions between articular cartilage and cationic antibodies, antigens, and immune complexes.
Charge-charge interactions between articular cartilage and cationic antibodies, antigens, and immune complexes.
复制标题
关节软骨与阳离子抗体、抗原和免疫复合物之间的电荷相互作用。
DOI：
10.1002/art.1780301109
复制
发表时间：
1987
影响因子：
--
通讯作者：
Mannik,M
中科院分区：
文献类型：
--
作者：
Zatarain-Rios,E;Mannik,M
用牛关节软骨通过电荷相互作用检测阳离子抗体、抗原和免疫复合体与关节软骨的结合。在兔抗人血清白蛋白抗体(抗-HSA)和人血清白蛋白抗体(HSA)中加入不同程度的氨基，使其阳离子化。当在人血清白蛋白中添加约20个或更多的新氨基，在抗人血清白蛋白中添加约25个或更多的氨基时，蛋白质很容易与软骨结合并渗透到基质中。与阳离子抗体形成的可溶性免疫复合体，包括小格子复合体，仅结合在软骨表面。当阳离子人血清白蛋白与软骨基质结合时，未改变的抗体仅与表面的抗原结合，不会穿透基质。这个模型系统定义了阳离子抗原和抗体结合和穿透关节软骨的方式。
Bovine articular cartilage was used to examine the binding of cationized antibodies, antigens, and immune complexes to articular cartilage by charge‐charge interactions. Rabbit antibodies to human serum albumin (anti‐HSA) and HSA were cationized to various degrees by the addition of amino groups. When approximately 20 or more new amino groups were added to HSA and approximately 25 or more amino groups were added to anti‐HSA, the proteins readily bound to cartilage and penetrated into the matrix. Soluble immune complexes made with the cationic antibodies, including small‐latticed complexes, bound only to the surface of the cartilage. When cationic HSA was bound to the matrix of the cartilage, unaltered antibodies bound only to the antigen at the surface and did not penetrate the matrix. This model system defines the manner in which cationic antigens and antibodies bind to and penetrate into the articular cartilage.