Rat HPC-1/syntaxin 1A and syntaxin 1B interrupt intracellular membrane transport and inhibit secretion of the extracellular matrix in embryonic cells of an amphibian.
Rat HPC-1/syntaxin 1A and syntaxin 1B interrupt intracellular membrane transport and inhibit secretion of the extracellular matrix in embryonic cells of an amphibian.
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大鼠 HPC-1/突触融合蛋白 1A 和突触融合蛋白 1B 会中断两栖动物胚胎细胞中的细胞内膜运输并抑制细胞外基质的分泌。
DOI：
10.1006/excr.1995.1347
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发表时间：
1995
影响因子：
3.7
通讯作者：
K. Akagawa
中科院分区：
文献类型：
--
作者：
S. Komazaki;T. Fujiwara;M. Takada;K. Akagawa
HPC-1/syntaxin 1A和syntaxin 1B是参与突触囊泡与神经细胞突触前质膜对接和/或融合的蛋白。将大鼠HPC-1和syntaxin 1B的带帽rna （cRNAs）注射到两栖动物日本蝾螈的胚胎细胞中。然后研究了从注射的crna翻译的蛋白质对细胞膜内运输和细胞外基质（ECM）分泌的影响。免疫印迹和免疫电镜显示，胚胎细胞中合成的HPC-1定位于高尔基复合物和液泡膜和质膜上。电镜观察显示，注射crna的胚胎细胞表面高尔基复合物形态变形，出现大量液泡，ECM消失。结果表明，HPC-1和syntaxin 1B阻断了两栖动物胚胎细胞的细胞膜内转运途径，抑制了ECM的分泌。哺乳动物神经细胞突触囊泡分泌过程的调节，以及两栖动物胚胎细胞胞内转运和ECM组成分泌的调节，可能涉及类似的机制。
HPC-1/syntaxin 1A and syntaxin 1B are proteins that have been implicated in the docking and/or fusion of synaptic vesicles to the presynaptic plasma membrane in neural cells. Capped RNAs (cRNAs) for rat HPC-1 and syntaxin 1B were injected into embryonic cells of an amphibian, the Japanese newt. The effects of the proteins translated from the injected cRNAs on intracellular membrane transport and secretion of the extracellular matrix (ECM) were then investigated. Immunoblotting and immunoelectron microscopy showed that the HPC-1 synthesized in the embryonic cells was localized on the membranes of Golgi complexes and vacuoles and on the plasma membrane. Electron microscopy revealed the morphological deformation of Golgi complexes, an appearance of large number of vacuoles, and the disappearance of the ECM from the cell surface in the cRNA-injected embryos. The results showed that HPC-1 and syntaxin 1B interrupt the pathways of intracellular membrane transport and inhibit the secretion of ECM by amphibian embryonic cells. Similar mechanisms may be involved in regulation of the secretory process of synaptic vesicles in mammalian neural cells and in regulation of intracellular membrane transport and constitutive secretion of ECM in amphibian embryonic cells.