Neuroprotective effects of Polygonum multiflorum on nigrostriatal dopaminergic degeneration induced by paraquat and maneb in mice
Neuroprotective effects of Polygonum multiflorum on nigrostriatal dopaminergic degeneration induced by paraquat and maneb in mice
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DOI：
10.1016/j.pbb.2005.09.004
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发表时间：
2005-10-01
影响因子：
3.6
通讯作者：
Kadota, S
中科院分区：
文献类型：
--
作者：
Li, X;Matsumoto, K;Kadota, S
在雄性 C57BL/6 小鼠中研究了何首乌提取物 (PME) 及其两种组分（乙醇可溶性 PME (PME-I) 和乙醇不溶性 PME (PME-II)）对百草枯和代森锰 (PQMB) 组合诱导的黑质纹状体多巴胺能神经元变性的神经保护作用。小鼠腹腔注射 PQMB，每周两次，持续 6 周。这种组合导致自发运动活动减少、运动不协调以及纹状体和黑质酪氨酸羟化酶阳性神经元的多巴胺水平下降。在 PQMB 治疗的 6 周期间和 PQMB 后的最后 8 天，每天一次连续 47 天施用 PME 和 PME-I 显着减轻了 PQMB 治疗动物的行为表现损伤以及纹状体多巴胺水平和黑质酪氨酸羟化酶阳性神经元的降低，而 PME-II 的施用对这些行为、神经化学和组织学指标没有影响。目前的研究结果表明，PME 对 PQMB 引起的帕金森病具有有益的影响，并且 PME 的作用可归因于 PME 的乙醇可溶部分（PME-I）中包含的某些物质。 (C) 2005 Elsevier Inc. 保留所有权利。
The neuroprotective effects of Polygonum multiflorum extract (PME) and its two fractions, ethanol-soluble PME (PME-I) and -insoluble PME (PME-II), on the degeneration of nigrostriatal dopaminergic neurons induced by a combination of paraquat and maneb (PQMB) were investigated in male C57BL/6 mice. The mice were treated twice a week for 6 weeks with intraperitoneal injections of PQMB. This combination caused a reduction of spontaneous locomotor activity, motor incoordination, and declines of dopamine level in the striatum and tyrosine hydroxylase-positive neurons in the substantia nigra. Administration of PME and PME-I once daily for 47 days during 6 weeks of PQMB treatment and last 8 days after PQMB significantly attenuated the impairment of behavioral performance and the decrease in striatal dopamine level and substantia nigral tyrosine hydroxylase-positive neurons in the PQMB-treated animals, whereas the administration of PME-II had no effect on these behavioral, neurochemical and histological indices. The present findings suggest that PME has a beneficial influence on parkinsonism induced by PQMB and that the effects of PME are attributable to some substance(s) included in the ethanol-soluble fraction of PME (PME-I). (C) 2005 Elsevier Inc. All rights reserved.