TRANSGENIC MICE AS A MODEL TO STUDY THE ROLE OF TGF-BETA-RELATED MOLECULES IN HAIR-FOLLICLES
TRANSGENIC MICE AS A MODEL TO STUDY THE ROLE OF TGF-BETA-RELATED MOLECULES IN HAIR-FOLLICLES
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DOI：
10.1101/gad.7.2.204
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发表时间：
1993-02-01
影响因子：
10.5
通讯作者：
HOGAN, BLM
中科院分区：
文献类型：
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作者：
BLESSING, M;NANNEY, LB;HOGAN, BLM
越来越多的证据表明，TGF-β超家族成员是体内上皮生长和分化的重要调节因子。在这里，转基因小鼠已被用于研究TGF-β相关的生长因子BMP-2和BMP-4在头发和胡须发育中的作用。在成熟的毛囊中，BMP-2转录本通常仅见于毛干基部的前皮质细胞中。在这里报道的转基因小鼠，BMP-4，一个密切相关的分子，已异位表达的外根鞘的头发和胡须毛囊使用的表达载体的基础上的牛细胞角蛋白IV（星星）启动子。响应于转基因表达，干细胞隔室下方的外根鞘细胞和毛乳头周围的毛基质细胞都停止增殖。此外，在一些转基因毛囊中，细胞角蛋白标记物的表达模式受到干扰。这些结果支持了一种模型，其中TGF-β超家族的成员在抑制细胞增殖和启动细胞特异性基因表达中发挥积极作用，所述细胞特异性基因表达发生在细胞离开卵泡基质并分化成干细胞时。
There is increasing evidence that members of the TGF-beta superfamily are important regulators of epithelial growth and differentiation in vivo. Here, transgenic mice have been used to study the role of the TGF-beta-related growth factors BMP-2 and BMP-4 in hair and whisker development. In the mature hair follicle, BMP-2 transcripts are normally seen only in precortex cells at the base of the hair shaft. In the transgenic mice reported here, BMP-4, a closely related molecule, has been ectopically expressed in the outer root sheath of hair and whisker follicles using an expression vector based on the bovine cytokeratin IV(star) promoter. In response to transgene expression, both outer root sheath cells below the stem cell compartment and hair matrix cells around the dermal papilla cease proliferation. In addition, the expression pattern of cytokeratin markers is disturbed in some transgenic hair follicles. These results support a model in which members of the TGF-beta superfamily play an active role in the inhibition of cell proliferation and the onset of expression of trichocyte-specific genes that take place when cells leave the matrix of the follicle and differentiate into shaft cells.