Cyclooxygenase-2 activity is important in craniofacial fracture repair
Cyclooxygenase-2 activity is important in craniofacial fracture repair
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DOI：
10.1016/j.ijom.2010.10.011
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发表时间：
2011-03-01
影响因子：
2.4
通讯作者：
Takato, T.
中科院分区：
文献类型：
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作者：
Chikazu, D.;Fujikawa, Y.;Takato, T.
本研究旨在探讨环氧合酶（考克斯）-2（COX-2）在小鼠颅面骨折后骨修复中的作用。在8周龄雄性考克斯-2野生型（考克斯-2（+/+））和敲除（考克斯-2（-/-））小鼠的顶骨中产生4 mm骨折。从骨折的骨头中提取了核糖核酸并进行了分析。为了进行形态学和组织学分析，在处理后8周和12周处死小鼠，并制备切片。进行三维计算机断层扫描，切片用苏木精-伊红染色进行组织学检查。在考克斯-2（+/+）小鼠中诱导了考克斯-2信使核糖核酸的表达，但在考克斯-2（-/-）小鼠中没有。考克斯-2（+/+）小鼠骨折后12周骨折部位的骨化几乎完全。在考克斯-2（-/-）小鼠中，骨折部位发生不完全愈合。在这两种类型的小鼠中，骨折部位不含软骨组织，骨痂从骨膜侧形成。这些结果表明，考克斯-2在颅面骨折修复中起着重要的作用，考克斯-2选择性非甾体类抗炎药可能会干扰骨折修复的膜脏颅在临床设置。
The aim of this study was to examine the effect of cyclooxygenase (COX)-2 on bone repair after craniofacial fracture in mice. A 4-mm fracture was created in the parietal bone of 8-week-old male COX-2 wild-type (COX-2(+/+)) and knockout (COX-2(-/-)) mice. Ribonucleic acid was extracted from the fractured bone and analysed. For morphological and histological analysis, the mice were killed 8 and 12 weeks after treatment, and sections were prepared. Three-dimensional computed tomography was performed, and the sections were stained with hematoxylin eosin for histological examination. Expression of COX-2 messenger ribonucleic acid was induced in COX-2(+/+) mice, but not in COX-2(-/-) mice. Ossification at the fracture site was almost complete 12 weeks after fracture in COX-2(+/+) mice. In COX-2(-/-) mice, incomplete union had occurred at the fracture site. In both types of mice, the fracture site contained no cartilaginous tissue, and the callus formed from the periosteal side. These results suggest that COX-2 plays an important role in craniofacial fracture repair and that COX-2-selective non-steroidal anti-inflammatory drugs might interfere with fracture repair of the membranous viscerocranium in the clinical setting.