A citrus flavonoid hesperidin suppresses infection-induced endotoxin shock in mice
A citrus flavonoid hesperidin suppresses infection-induced endotoxin shock in mice
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DOI：
10.1248/bpb.27.679
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发表时间：
2004-05-01
影响因子：
2
通讯作者：
Kumazawa, Y
中科院分区：
文献类型：
--
作者：
Kawaguchi, K;Kikuchi, S;Kumazawa, Y
在LPS攻击前给予小鼠柑橘类黄酮橙皮苷（HES）以剂量依赖性方式显著降低肿瘤坏死因子（TNF）-α的产生。腹膜内（i. p.）用10（8）CFU鼠伤寒沙门氏菌aroA感染导致从致死性休克中拯救，与LPS无应答小鼠相似。羟乙基淀粉预处理不仅能显著降低肝脏和脾脏中的细菌数量，还能显著降低血浆中的LPS水平。此外，羟乙基淀粉显著抑制了TNF-α和高迁移率族蛋白1（HMGB-1）的血浆水平，减少了肝脏中凋亡细胞的数量，并使凝血因子的活化状态正常化，如凝血酶原时间和感染引起的血小板数量。LPS与HES预处理抑制显色鲎反应。
Administration of a citrus flavonoid hesperidin (HES) to mice before LPS challenge significantly reduced tumor necrosis factor (TNF)-alpha production in a dose-dependent manner. Treatment of HES 3 h before intraperitoneal (i.p.) infection with 10(8) CFU Salmonella typhimurium aroA resulted in rescue from lethal shock as similar to LPS-nonresponder mice. Not only bacterial numbers in livers and spleens but also plasma LPS levels significantly decreased by pretreatment with HES. In addition, HES markedly suppressed plasma levels of TNF-alpha and high mobility group box chromosomal protein 1 (HMGB-1), decreased the number of apoptotic cells in livers and normalized the activated states of blood coagulation factors such as prothrombin time and platelet numbers caused by infection. Pretreatment of LPS with HES suppressed the chromogenic Limulus reaction.