Photocleavage of DNA: irradiation of quinone-containing reagents converts supercoiled to linear DNA.
Photocleavage of DNA: irradiation of quinone-containing reagents converts supercoiled to linear DNA.
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DNA 的光裂解：含醌试剂的照射将超螺旋 DNA 转化为线性 DNA。
DOI：
10.1111/j.1751-1097.1993.tb04931.x
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发表时间：
1993
影响因子：
3.3
通讯作者：
Schuster,GB
中科院分区：
文献类型：
--
作者：
Koch,T;Ropp,JD;Sligar,SG;Schuster,GB
含有连接到季烷基铵基团的蒽醌基团的试剂的空气饱和溶液的照射（350 nm）将超螺旋DNA转化为环状和线性DNA。线性DNA的产生不是通过大量单链切割的积累，而是通过DNA的重合位点双链切割发生的。辐射形成蒽醌的三重态，其通过从DNA的糖提取氢原子或通过从DNA的碱基转移电子而反应。随后的反应导致断链。在此序列之后，醌显然重新形成，并且再照射导致双链裂解。
Irradiation (350 nm) of air‐saturated solutions of reagents containing an anthraquinone group linked to quaternary alkyl ammonium groups converts supercoiled DNA to circular and to linear DNA. Generation of linear DNA does not occur by accumulation of numerous single‐strand cuts but by coincident‐site double‐strand cleavage of DNA. Irradiation forms the triplet state of the anthraquinone, which reacts either by hydrogen atom abstraction from a sugar of DNA or by electron transfer from a base of the DNA. Subsequent reactions result in chain scission. The quinone is apparently reformed after this sequence and reirradiation leads to double‐strand cleavage.