The mechanism of antigen-presentation of avian bone marrowed dendritic cells suppressed by infectious bronchitis virus
The mechanism of antigen-presentation of avian bone marrowed dendritic cells suppressed by infectious bronchitis virus
复制标题
传染性支气管炎病毒抑制禽骨髓树突状细胞抗原呈递机制
DOI：
10.1016/j.ygeno.2021.04.027
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发表时间：
2021
期刊：
影响因子：
4.4
通讯作者：
Qian Yang
中科院分区：
文献类型：
--
作者：
Jinjiao Zuo;Yanan Cao;Zhisheng Wang;Abid Ullah Shah;Wenlei Wang;Chen Dai;Mingjia Chen;Jian Lin;Qian Yang
树突状细胞是抵御禽传染性支气管炎病毒（IBV）感染和侵袭的第一道防线。而IBV总是抑制树突状细胞并逃避降解和呈递，这可能有助于病毒的转移和迁移。首先，我们比较了两种IBV对禽类骨髓树突状细胞（bmdc）的激活功能，发现IBV （QX和M41）均未显著增加禽类骨髓树突状细胞的表面标记物。此外，在IBV （QX）流行感染的禽类BMDCs中，m6A mRNA修饰水平显著降低，但ut RNA修饰水平升高。进一步研究发现，非结构蛋白7 （NSP7）和NSP16均能抑制BMDCs的成熟和细胞因子分泌，并抑制其抗原呈递能力。最后，我们发现NSP7和NSP16诱导的gga-miR21抑制了禽类BMDCs的抗原呈递。综上所述，我们的结果说明了IBV如何抑制禽dc的抗原呈递。
Dendritic cells are first guard to defend avian infectious bronchitis virus (IBV) infection and invasion. While IBV always suppress dendritic cells and escape the degradation and presentation, which might help viruses to transfer and migrant. Initially, we compared two IBV's function in activating avian bone marrow dendritic cells (BMDCs) and found that both IBV (QX and M41) did not significantly increase surface marker of avian BMDCs. Moreover, a significant decrease of m6A modification level in mRNA, but an increased in the ut RNA were observed in avian BMDCs upon the prevalent IBV (QX) infection. Further study found that both non-structural protein 7 (NSP7) and NSP16 inhibited the maturation and cytokines secretion of BMDCs, as well as their antigen-presentation ability. Lastly, we found that gga-miR21, induced by both NSP7 and NSP16, inhibited the antigen presentation of avian BMDCs. Taken together, our results illustrated how IBV inhibited the antigen-presentation of avian DCs.