Genome-wide non-mendelian inheritance of extra-genomic information in Arabidopsis
Genome-wide non-mendelian inheritance of extra-genomic information in Arabidopsis
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DOI：
10.1038/nature03380
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发表时间：
2005-03-24
期刊：
影响因子：
64.8
通讯作者：
Pruitt, RE
中科院分区：
文献类型：
--
作者：
Lolle, SJ;Victor, JL;Pruitt, RE
经典孟德尔遗传学的一个基本原则是等位基因信息稳定地从一代传到下一代，导致不同等位基因的可预测分离模式(1)。虽然这一原则有几个例外，但都是机械地限制在有限的一组特定基因(例如酵母中的交配型转换(2))或特定类型的等位基因(例如包含转座子(3)或重复序列(4)的等位基因)的特殊情况。在这里，我们证明了器官融合基因Hethead(5)(HTH)的隐性突变等位基因纯合的拟南芥植物可以遗传到亲本染色体基因组中没有但在前几代中存在的等位基因特异的DNA序列信息。这一先前未被描述的过程被证明在所有被检查的DNA序列多态中都发生，因此似乎是DNA序列信息的基因组外遗传的一般机制。我们推测，这些基因恢复事件是模板导向的过程的结果，该过程利用了祖先的RNA序列缓存。
A fundamental tenet of classical mendelian genetics is that allelic information is stably inherited from one generation to the next, resulting in predictable segregation patterns of differing alleles(1). Although several exceptions to this principle are known, all represent specialized cases that are mechanistically restricted to either a limited set of specific genes ( for example mating type conversion in yeast(2)) or specific types of alleles ( for example alleles containing transposons(3) or repeated sequences(4)). Here we show that Arabidopsis plants homozygous for recessive mutant alleles of the organ fusion gene HOTHEAD(5) (HTH) can inherit allele-specific DNA sequence information that was not present in the chromosomal genome of their parents but was present in previous generations. This previously undescribed process is shown to occur at all DNA sequence polymorphisms examined and therefore seems to be a general mechanism for extragenomic inheritance of DNA sequence information. We postulate that these genetic restoration events are the result of a template-directed process that makes use of an ancestral RNA-sequence cache.