Estrogen Receptor α Gene Promoter 0/B Usage in the Rat Sexually Dimorphic Nucleus of the Preoptic Area
Estrogen Receptor α Gene Promoter 0/B Usage in the Rat Sexually Dimorphic Nucleus of the Preoptic Area
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DOI：
10.1210/en.2009-1022
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发表时间：
2010-04-01
期刊：
影响因子：
4.8
通讯作者：
Sakuma, Yasuo
中科院分区：
文献类型：
--
作者：
Hamada, Tomohiro;Sakuma, Yasuo
雄性大鼠视前区性二形核（SDN-POA）的体积是雌性大鼠的两到四倍；然而，两性二态性的建立机制和该核的功能几乎未知。围产期雌激素可通过雌激素受体α（ERα）引起性别二态性。最近，转基因大鼠在 ER α 基因启动子 0/B 的控制下表达增强型绿色荧光蛋白 (EGFP)，以标记大脑中 ER α 阳性神经元。在本研究中，我们检查了这种 EGFP 表达是否可以作为成人 SDN-POA 的标记。根据尼氏染色和 SDN 标记物钙结合蛋白的免疫反应性，EGFP 标记的细胞分布在雄性和雌性转基因大鼠的 SDN-POA (0/B-SDN) 核心。它们对 ER α 也有免疫反应。男性的核心体积比女性更大，并且含有更多的 0/B-SDN 神经元。雌性核心细胞中 EGFP 标记的细胞比雄性细胞更密集。在女性出生当天皮下注射100μg 17β-雌二醇，或对男性新生儿进行睾丸切除术，尽管不影响细胞数量，但逆转了0/B-SDN体积的性别二态性表型。我们认为 SDN-POA 中的 EGFP 表达可能是阐明 SDN-POA 性别分化和功能的有用标记。此外，ERα基因启动子0/B在SDN-POA性别分化的组织中起着关键作用。 （内分泌学151：1923-1928，2010）
The volume of the sexually dimorphic nucleus of the preoptic area (SDN-POA) is two to four times larger in male rats than in females; however, the mechanism for the establishment of sexual dimorphism and the function of this nucleus is almost unknown. Perinatal estrogen can cause sexual dimorphism via the estrogen receptor alpha (ER alpha). Recently, transgenic rats were generated that express enhanced green fluorescent protein (EGFP) under the control of the ER alpha gene promoter 0/B to tag ER alpha-positive neurons in the brain. In the present study, we examined whether this EGFP expression could be a marker for the SDN-POA in adults. EGFP-labeled cells were distributed in the core of the SDN-POA (0/B-SDN)of male and female transgenic rats, in accordance with the Nissl staining and immunoreactivity for the SDN marker, calbindin. They were also immunoreactive for ER alpha. The core was bigger in volume and contained more 0/B-SDN neurons in males than in females. The EGFP-tagged cells were packed more densely in the female core than that in males. Subcutaneous injection of 100 mu g 17 beta-estradiol to females on the day of birth, or orchidectomy of male neonates, reversed the sexually dimorphic phenotype of the volume of the 0/B-SDN, despite not affecting the cell number. We suggest that this EGFP expression in the SDN-POA could be a useful marker to clarify the sexual differentiation and function of the SDN-POA. Moreover, the ER alpha gene promoter 0/B plays a key role in the organization of the sexual differentiation of the SDN-POA. ( Endocrinology 151: 1923-1928, 2010)