Real-Time Monitoring of Oxidative Burst from Single Plant Protoplasts Using Microelectrochemical Sensors Modified by Platinum Nanoparticles
Real-Time Monitoring of Oxidative Burst from Single Plant Protoplasts Using Microelectrochemical Sensors Modified by Platinum Nanoparticles
复制标题
使用铂纳米粒子修饰的微电化学传感器实时监测单植物原生质体的氧化爆发
DOI：
10.1021/ac901300b
复制
发表时间：
2009-10-15
影响因子：
7.4
通讯作者：
Huang, Wei-Hua
中科院分区：
文献类型：
--
作者：
Ai, Feng;Chen, Hong;Huang, Wei-Hua
植物的氧化爆发在植物抗病和信号转导中起着重要的作用，然而，迄今为止，它还没有在单个活的植物细胞上进行过研究。在这篇文章中，我们制作了一种新的敏感的电化学传感器的基础上，电化学沉积的Pt纳米粒子的碳纤维微盘电极的表面上通过一个纳米孔含有聚合物基体，民族。在电极表面包覆的Nafion簇中，大量的亲水性纳米通道作为Pt的沉积和分散的分子模板，导致了小Pt纳米颗粒的均匀构建。该传感器对H_2O_2具有很高的检测灵敏度，检测限为5.0 × 10 ~（-9）M。利用该微电化学传感器，首次实现了对植物原生质体氧化猝发的实时监测。结果表明，病原菌类似物引发的单原生质体氧化爆发具有量子释放的特征，伴随着大量的“瞬时氧化微爆发”。结果表明，转基因植株的原生质体在生物学上表现出更好的抗病性，并表现出显著的升高和更持久的氧化爆发。
Oxidative bursts from plants play significant roles in plant disease defense and signal transduction; however, it has not hitherto been investigated on individual living plant cells. In this article, we fabricated a novel sensitive electrochemical sensor based on electrochemical deposition of Pt nanoparticles on the surface of carbon fiber microdisk electrodes via a nanopores containing polymer matrix, Nation. The numerous hydrophilic nanochannels in the Nafion clusters coated on the electrode surface served as the molecular template for the deposition and dispersion of Pt, which resulted in the uniform construction of small Pt nanoparticles. The novel sensor displayed a high sensitivity for detection of H2O2 with a detection limit of 5.0 x 10(-9) M. With the use of this microelectrochemical sensor, the oxidative burst from individual living plant protoplasts have been real-time monitored for the first time. The results showed that oxidative burst from single protoplasts triggered by a pathogen analogue were characterized by quanta release with a large number of "transient oxidative microburst!' events, and protoplasts from the transgenic plants biologically displayed better disease-resistance and showed a distinguished elevation and longer-lasting oxidative burst.