Parallelized label-free detection of protein interactions using a hyper-spectral imaging system
Parallelized label-free detection of protein interactions using a hyper-spectral imaging system
复制标题
使用高光谱成像系统并行无标记检测蛋白质相互作用
DOI：
10.1039/c5ay00738k
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
S. Jiang M. Iga and E. Tamiya
中科院分区：
文献类型：
--
作者：
H. Yoshikawa;M. Murahashi; M. Saito;S. Jiang M. Iga and E. Tamiya
我们描述了并行化的无标记检测蛋白质的相互作用，使用高光谱成像系统。通过将抗体固定在Au帽纳米柱聚合物膜上来制造多阵列蛋白质芯片，所述Au帽纳米柱聚合物膜在吸收光谱中由于局部表面等离子体共振（LSPR）而显示出光学吸收带。通过处理应用抗原之前和之后的多阵列芯片的高光谱图像，证明了多种抗原-抗体相互作用的标记化检测。人免疫球蛋白A（伊加）和人C-反应蛋白（CRP）的特异性检测，并获得对应于这些蛋白质浓度的信号，表明该技术是有前途的，用于提高性能的多个蛋白质测定。
We describe parallelized label-free detection of protein interactions using a hyper-spectral imaging system. Multi-array protein chips were fabricated by immobilizing antibodies on Au-capped nanopillar polymer films, which showed an optical absorption band due to the localised surface plasmon resonance (LSPR) in the absorption spectrum. Parallelized detection of multiple antigen–antibody interactions was demonstrated by processing hyper-spectral images of the multi-array chip before and after the application of antigens. Human immunoglobulin A (IgA) and human C-reactive protein (CRP) were specifically detected, and the signal corresponding to these protein concentrations was obtained, indicating that this technique is promising for use in enhancing the performance of multiple protein assays.