Sensory and sympathetic innervation of the mammalian cornea. A retrograde tracing study.
Sensory and sympathetic innervation of the mammalian cornea. A retrograde tracing study.
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DOI：
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发表时间：
1989-03
影响因子：
4.4
通讯作者：
Carl F. Marfurr;R. E. Kingsley;Stephen E. Echtenkamp
中科院分区：
文献类型：
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作者：
Carl F. Marfurr;R. E. Kingsley;Stephen E. Echtenkamp
应用辣根过氧化物酶-麦胚凝集素（HRP-WGA）逆行追踪法，研究了4种哺乳动物角膜传入和传出神经元的分布、数目和胞体直径。将2%的HRP-WGA溶液应用于大鼠、兔、猫和猴的中央角膜，并在48-96小时后灌注固定动物。HRP-WGA标记的感觉神经元在大鼠、猫和猴同侧三叉神经节的眼区分布相对均匀。与此形成鲜明对比的是，标记细胞在兔三叉神经节聚集在一个明确的纵向柱位于中央区的眼区。在一些猫和猴中，在神经节的上颌区域附近（可能在该区域内）偶尔观察到细胞。没有证据表明任何物种的角膜传入神经元的背腹侧躯体解剖学。大多数标记的传入胞体的大小是小或中等，虽然也观察到一些较大直径的神经元。在兔和猫的同侧上级颈神经节（SCG）中观察到中等数量的标记神经元;然而，在大鼠的SCG中仅偶尔观察到标记神经元，在猴中未观察到标记神经元。标记的SCG细胞，当存在时，集中在吻侧的一半的神经节，虽然在猫SCG中的许多细胞也被发现进一步尾部。在所有动物的颈中神经节或星状神经节中均未发现标记神经元。这项研究的结果揭示了哺乳动物角膜传入和传出神经支配的组织存在微妙的种间差异。
The method of retrograde transport of horseradish peroxidase-wheat germ agglutinin (HRP-WGA) was used to study the locations, numbers and somata diameters of corneal afferent and efferent neurons in four different mammalian species. A 2% solution of HRP-WGA was applied to the central cornea of the rat, rabbit, cat and monkey and the animals were perfusion-fixed 48-96 hr later. HRP-WGA-labeled sensory neurons were distributed relatively uniformly throughout the ophthalmic region of the ipsilateral trigeminal ganglion of the rat, cat and monkey. In marked contrast, labeled cells in the rabbit trigeminal ganglion were clustered in a sharply defined longitudinal column located in the midregion of the ophthalmic area. Occasional cells in some cats and monkeys were observed near, and possibly within, the maxillary region of the ganglion. There was no evidence for a dorsoventral somatotopy of corneal afferent neurons in any species. The majority of the labeled afferent somata were small or medium in size, although some larger diameter neurons were also observed. Modest numbers of labeled neurons were observed in the ipsilateral superior cervical ganglion (SCG) of the rabbit and cat; however, only occasional labeled neurons were observed in the SCG of the rat, and none were seen in the monkey. The labeled SCG cells, when present, were concentrated in the rostral half of the ganglion, although many cells in the cat SCG were also found further caudally. No labeled neurons were found in the middle cervical or stellate ganglia of any animal. The results of this study have revealed the existence of subtle interspecies differences in the organization of the mammalian corneal afferent and efferent innervations.