A gene expression programme induced by bovine colostrum whey promotes growth and wound-healing processes in intestinal epithelial cells
A gene expression programme induced by bovine colostrum whey promotes growth and wound-healing processes in intestinal epithelial cells
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DOI：
10.1017/jns.2014.56
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Lessard, M.
中科院分区：
文献类型：
--
作者：
Blais, M.;Pouliot, Y.;Lessard, M.
牛初乳因其有益健康和发育的特性而广为人知。它含有各种各样的生物活性成分，已知这些成分可以促进许多细胞过程。因此，人们建议使用初乳乳清作为饲料添加剂来促进肠道健康，但对其对肠道上皮细胞有益的作用机制却知之甚少。本论文从牛初乳中去除酪蛋白，并用剩余的富含生物活性物质的乳清对猪肠道上皮细胞系IPEC-J2和人结肠腺癌细胞系Caco-2/15的细胞过程进行调控。首先，我们验证了初乳乳清和奶酪乳清对肠道创伤愈合过程的影响，包括细胞的增殖、附着、形态和迁移。结果表明，初乳乳清对Caco-2/15细胞的增殖和迁移有促进作用，对Caco-2/15细胞在培养皿上的黏附有明显的抑制作用。另一方面，奶酪乳清可诱导IPEC-J2细胞增殖和形态改变，但不能诱导细胞迁移。应用基因芯片技术分析初乳乳清处理后IPEC-J2细胞的基因表达谱。结果表明，在初乳乳清处理的细胞中，涉及细胞迁移、黏附和增殖的大量基因的表达确实受到影响。总而言之，初乳特定的生物活性成分可能通过精确的基因表达程序控制与伤口愈合有关的生物过程，从而有利于肠道上皮细胞的动态平衡。
Bovine colostrum is well known for its beneficial properties on health and development. It contains a wide variety of bioactive ingredients that are known to promote a number of cellular processes. Therefore the use of colostrum whey as a feed additive to promote intestinal health has been proposed, yet little is known about mechanisms implicated in its beneficial properties on intestinal epithelial cells. In the present paper, casein were removed from bovine colostrum and the remaining liquid, rich in bioactive compounds, was evaluated for its capacity to modulate cellular processes in porcine intestinal epithelial cell line IPEC-J2 and human colon adenocarcinoma cell line Caco-2/15. First, we verified the effect of colostrum whey and cheese whey on processes involved in intestinal wound healing, including cell proliferation, attachment, morphology and migration. Our results showed that colostrum whey promoted proliferation and migration, and decreased specifically the attachment of Caco-2/15 cells on the culture dish. On the other hand, cheese whey induced proliferation and morphological changes in IPEC-J2 cells, but failed to induce migration. The gene expression profile of IPEC-J2 cells following colostrum whey treatment was evaluated by microarray analysis. Results revealed that the expression of a significant number of genes involved in cell migration, adhesion and proliferation was indeed affected in colostrum whey-treated cells. In conclusion, colostrum specific bioactive content could be beneficial for intestinal epithelial cell homoeostasis by controlling biological processes implicated in wound healing through a precise gene expression programme.