Activated leucocytes express and secrete macrophage inflammatory protein-1α upon interaction with synovial fibroblasts of rheumatoid arthritis via a β2-integrin/ICAM-1 mechanism
Activated leucocytes express and secrete macrophage inflammatory protein-1α upon interaction with synovial fibroblasts of rheumatoid arthritis via a β2-integrin/ICAM-1 mechanism
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DOI：
10.1093/rheumatology/keg391
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发表时间：
2003-11-01
期刊：
影响因子：
5.5
通讯作者：
Adachi, M
中科院分区：
文献类型：
--
作者：
Hanyuda, M;Kasama, T;Adachi, M
客观的。检测从类风湿性关节炎（RA）患者滑液（SF）中分离的成纤维样滑膜细胞（FLS）、单核细胞和多形核中性粒细胞（PMN）对趋化因子、巨噬细胞炎症蛋白-1α（MIP-1α）的表达和调节。方法。从 RA SF 获得的单核细胞或 PMN 与未刺激的半汇合 RA FLS 共培养。通过酶联免疫吸附测定法测定培养物上清液中的 MIP-1α。分别通过Northern印迹分析和免疫组化检测MIP-1α mRNA和蛋白的表达。结果。活化的白细胞与 FLS 的相互作用通过 β(2)-整合素/细胞间粘附分子介导的机制协同增加 MIP-1α 的表达和分泌 1.结论。发炎关节内的 MIP-1α 表达似乎不仅受到炎症细胞因子的调节，还受到活化白细胞和 FLS 的物理相互作用的调节，并且在 RA 滑膜炎的进展和维持中发挥着至关重要的作用。
Objective. To examine the expression and regulation of chemotactic factor, macrophage inflammatory protein-1alpha (MIP-1alpha) by fibroblast-like synoviocytes (FLS), monocytes and polymorphonuclear neutrophils (PMN) isolated from the synovial fluid (SF) of rheumatoid arthritis (RA) patients.Methods. Monocytes or PMN obtained from RA SF were co-cultured with unstimulated semiconfluent RA FLS. Culture supernatants were assayed for MIP-1alpha by enzyme-linked immunosorbent assay. The expression of MIP-1alpha mRNA and protein was also determined by Northern blot analyss and immunohistochemistry respectively.Results. Interaction of activated leucocytes with FLS synergistically increased MIP-1alpha expression and secretion via a mechanism mediated by beta(2)-integrin/ intercellular adhesion molecule 1.Conclusion. MIP-1alpha expression within inflamed joints appears to be regulated not only by inflammatory cytokines but also by the physical interaction of activated leucocytes and FLS, and plays a crucial role in the progression and maintenance of RA synovitis.