Immunization with human thyrotrophin receptor peptide induces an increase in thyroid hormone in rabbits.
Immunization with human thyrotrophin receptor peptide induces an increase in thyroid hormone in rabbits.
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用人促甲状腺素受体肽免疫可诱导兔子体内甲状腺激素增加。
DOI：
10.1677/joe.0.1350479
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
T. Onaya
中科院分区：
文献类型：
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作者：
M. Ohmori;T. Endo;M. Ikeda;T. Onaya
用与人促甲状腺激素(TSH)受体胞外区N末端区域(称为N肽，氨基酸残基29-57)对应的合成肽免疫8只兔。10周后，8只兔全部产生抗N肽抗体。Western印迹分析表明，该抗体识别兔TSH受体为约100 kDa的蛋白。我们比较了免疫前和免疫后血清中甲状腺激素的水平。8只兔的免疫后血清具有较高的均值(+/-S.D.)三碘甲腺原氨酸(T_3)和甲状腺激素(T_4)水平高于免疫前血清(T_3，前免疫0.82+/-0.26微克/L vs免疫后1.33+/-0.35，P<；0.01；T_4，前免疫33.7+/-10.0微克/L vs免疫后41.0+/-6.0，P<；0.05)。免疫后4只兔的T_3水平和4只兔的T_4水平都超过了6只年龄匹配的对照兔的正常范围。7只兔有不同程度的甲状腺刺激性抗体(TSAb)活性(241-545%)。T_3、T_4在非免疫组(T_3，前免疫0.89+/-0.34微克/L比免疫后0.82+/-0.22；T_4，前免疫31.1+/-7.3微克/L比免疫后30.3+/-5.1)和其他多肽免疫组(T_3，前免疫0.68微克/L(n=2)比免疫后0.69；T_4，前免疫33.1微克/L比免疫后26.4)在10周内没有增加。这些结果表明，实验产生的具有TSAb活性的抗TSH受体抗体可诱导兔甲状腺激素的增加。
Eight rabbits were immunized with a synthetic peptide corresponding to the unique N-terminal region (termed N peptide; amino acid residues 29-57) in the extracellular domain of the human thyrotrophin (TSH) receptor. After 10 weeks, all of the eight rabbits produced anti-N peptide antibodies. Western blot analysis revealed that the antibodies recognized rabbit TSH receptor as an approximately 100 kDa protein. We compared the level of thyroid hormone in serum taken before immunization (preimmune sera) with that of serum taken after immunization (postimmune sera) in these immunized rabbits. Postimmune sera from the eight rabbits had higher mean (+/- S.D.) levels of tri-iodothyronine (T3) and thyroxine (T4) than did preimmune sera (T3, preimmune 0.82 +/- 0.26 micrograms/l vs postimmune 1.33 +/- 0.35, P < 0.01; T4, preimmune 33.7 +/- 10.0 micrograms/l vs postimmune 41.0 +/- 6.0, P < 0.05). T3 levels in four rabbits and T4 levels in four rabbits after immunization were over the normal range obtained from six age-matched control rabbits. Seven rabbits exhibited thyroid-stimulating antibody (TSAb) activity with various degrees (241-545%). The concentration of T3 and T4 did not increase over 10 weeks in either non-immunized rabbits (T3, preimmune 0.89 +/- 0.34 micrograms/l vs postimmune 0.82 +/- 0.22; T4, preimmune 31.1 +/- 7.3 micrograms/l vs postimmune 30.3 +/- 5.1) or other peptide-immunized rabbits (T3, preimmune 0.68 micrograms/l (n = 2) vs postimmune 0.69; T4, preimmune 33.1 micrograms/l vs postimmune 26.4). These results indicate that experimentally produced anti-TSH receptor antibody with TSAb activity induces an increase in thyroid hormone in rabbits.