Distribution of the collagen IV isoforms in human Bruch's membrane
Distribution of the collagen IV isoforms in human Bruch's membrane
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DOI：
10.1136/bjo.87.2.212
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发表时间：
2003-02-01
影响因子：
4.1
通讯作者：
Handa, JT
中科院分区：
文献类型：
--
作者：
Chen, L;Miyamura, N;Handa, JT
目的：研究IV型胶原蛋白α 1 - α 6链在人后极Bruch膜中的分布。方法：对18只眼（20个月至83岁）的冷冻切片（10只）进行酸处理，用10%正常山羊血清阻断，用抗IV型胶原异构体特异性肽的单克隆抗体（1:75稀释）孵育1小时，用ABC染色试剂盒观察。结果：在Bruch膜中，视网膜色素上皮（10/ 18 = 55%）和绒毛膜基底膜（18/18 = 100%）中检测到alpha1 （IV）和alpha2（IV）链；在视网膜色素上皮基底膜中也发现了alpha3（IV）、alpha4（IV）和alpha5（IV）链（13/18 = 72%）。脉络膜血管中检测到α 1 （IV）和α 2（IV）链（18/18 = 100%）。在任何切片中未发现α (IV)链。结论：α 1 -2（IV）和α 3-5（IV）在Bruch膜上的异质性分布可以揭示该结构在健康、衰老和年龄相关性黄斑变性等疾病中的功能。
Aims: To determine the distribution of the alpha1 to alpha6 chains of type IV collagen in Bruch's membrane of the human posterior pole.Methods: Cryosections (10 mum) from 18 human eyes (20 months to 83 years old) were acid treated, blocked with 10% normal goat serum, incubated for 1 hour with monoclonal antibodies against type IV collagen isoform specific peptides at 1:75 dilution, and visualised with an ABC staining kit.Results: In Bruch's membrane, the alpha1 (IV) and alpha2(IV) chains were identified in retinal pigment epithelial (10/ 18 = 55%) and choriocapillaris basement membranes (18/18 = 100%); the alpha3(IV), alpha4(IV), and alpha5(IV) chains were also found in the retinal pigment epithelial basement membrane (13/18 = 72%). In the choroid, the alpha1 (IV) and alpha2(IV) chains were detected in the blood vessels (18/18 = 100%). The alpha(IV) chain was not identified in any sections.Conclusion: The heterogeneous distribution of alpha1 -2(IV) and alpha3-5(IV) in Bruch's membrane could give insights into the function of this structure in health, ageing, and diseases such as age related macular degeneration.