Use of the traditional Chinese medicine “compound healthy ear agent” to protect against age-related hearing loss in mice: A proteomics study
Use of the traditional Chinese medicine “compound healthy ear agent” to protect against age-related hearing loss in mice: A proteomics study
复制标题
DOI：
10.1016/j.heliyon.2024.e26914
复制
发表时间：
2024-02
期刊：
影响因子：
4
通讯作者：
Weijun Xuan;Liyi Huang;Yi Xuan;Sizhong Chen;Junbo Tang;Yulong Wei;Xu Pan;Michael R. Hamblin
中科院分区：
文献类型：
--
作者：
Weijun Xuan;Liyi Huang;Yi Xuan;Sizhong Chen;Junbo Tang;Yulong Wei;Xu Pan;Michael R. Hamblin
背景研究表明中药复方健耳剂（compound healthyear agent，CHEA）具有抗耳蜗毛细胞和螺旋神经节细胞凋亡的作用，对老年性聋或年龄相关性听力损失（age-related hearing loss，阿勒）小鼠听力有保护作用，对氨基糖苷类抗生素耳毒性也有保护作用。方法将18只1月龄C57 BL/6 J小鼠随机分为3组：（A）饮水至2月龄（K2 M），（B）饮水至7月龄（K7 M），（B）饮水至2月龄（K2 M），（C）饮水至7月龄（K7 M），（D）饮水至2月龄（K2 M），（C）饮水至7月龄（K7 M），（D）饮水至2月龄（K2 M），（D）饮水至7月龄（K7 M）。（C）每天饮用含CHEA的水，直至7月龄为治疗组，Z7 M。结果假发现率（FDR）< 1%的蛋白质定性统计为5873个，定量统计为5492个。K7 M组与K2 M组、Z7 M组与K7 M组、Z7 M组与K2 M组差异富集至少1.5倍（p < 0.05）的蛋白数量分别为351个、52个、264个。Z7 M组差异表达的蛋白质涉及突触分子传递、能量代谢、免疫应答、抗氧化防御和抗凋亡等方面。结论中药CHEA通过调节耳蜗毛细胞和螺旋神经节神经元特异性蛋白和基因的表达，对小鼠阿勒具有保护作用。除了预期参与的途径（抗氧化和抗凋亡），与免疫反应相关的蛋白质是本研究的新发现。
BackgroundPrevious studies have shown that the traditional Chinese medicine (TCM) called "compound healthy ear agent" (CHEA) had anti-apoptosis effects in cochlear hair cells and spiral ganglion neurons, and could protect mice hearing against presbycusis or age-related hearing loss (AHL), as well as aminoglycoside antibiotic-induced ototoxicity. Because its mechanisms of action are still unclear, we investigated the mechanism of action of CHEA against AHL in mice using proteomics techniques.MethodsEighteen C57BL/6J mice at 1 month of age were randomly divided into three groups: (A) drinking water until 2 months of age, K2M); (B) drinking water until 7 months of age to induce AHL, K7M; (C) drinking water containing CHEA daily until 7 months of age as treatment group, Z7M. At 2 or 7 months mice were sacrificed and their cochleae were removed for proteomics analysis.ResultsThe numbers of proteins with a false discovery rate (FDR) < 1% were respectively 5873 for qualitative and 5492 for quantitative statistics. The numbers of proteins with differential enrichment at least 1.5-fold (p < 0.05) were respectively 351 for K7M vs K2M groups, 52 for Z7M vs K7M groups, 264 for Z7M vs K2M groups. The differentially expressed proteins in the Z7M group were involved in synaptic molecular transmission, energy metabolism, immune response, antioxidant defenses, and anti-apoptosis.ConclusionThe TCM CHEA played a protective role against AHL in mice by regulating the expression of specific proteins and genes in cochlear hair cells and spiral ganglion neurons. Besides the pathways expected to be involved (antioxidant and anti-apoptosis), proteins related to immune response is a new finding of the present study.