Differential expression of Homer family proteins in the developing mouse brain
Differential expression of Homer family proteins in the developing mouse brain
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DOI：
10.1002/cne.20116
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发表时间：
2004-06-07
影响因子：
2.5
通讯作者：
Furuichi, T
中科院分区：
文献类型：
--
作者：
Shiraishi, Y;Mizutani, A;Furuichi, T
Homer作为突触后衔接蛋白，连接多个靶点，如参与谷氨酸受体信号传导的蛋白质。我们报告的差异表达的长形式的荷马蛋白产生的三个独特的基因在出生后发育的小鼠大脑。Homer 1 B/c和Cupidin/Homer 2a/B广泛分布于整个发育中的大脑，并且在后脑起源区域如小脑、脑桥和延髓中下调。相比之下，Homer 3a/B仅限于小脑、海马和新生儿嗅球。在小脑中，Homer 1 B/c和Cupidin/Homer 2a/B在发育中的颗粒细胞的突触后占主导地位，而Homer 3a/B集中在浦肯野细胞的树突棘及其轴突中。Homer 1 B/c和Cupidin/Homer 2a/B的下调与Homer 3a/B在出生后第一周和第二周之间的上调形成鲜明对比。在海马中，Homer 1 B/c和Cupidin/Homer 2a/B分别主要分布在CA 1区和CA 1-CA 2区，而Homer 3a/B主要分布在CA 2-CA 3区，并在出生后第3周左右达到峰值。在海马细胞培养物中，Homer 1 B/c和Cupidin/Homer 2a/B在抑制性和兴奋性神经元中表达，而Homer 3a/B主要在兴奋性神经元中表达，但在抑制性神经元中不表达。在发育中的嗅球中，Homer 1 B/c和Cupidin/Homer 2a/B在颗粒层、外网状层和肾小球层中上调，而Homer 3a/B在出生后第一周内在这些区域中急剧降低。Cupidin/Homer 2a/B也在不同的嗅觉上皮区内的嗅觉感觉神经元中表达，然后广泛分布于嗅觉神经层中的轴突和嗅觉上皮中的纤毛。这些结果表明，荷马家族成员有不同的区域，细胞和亚细胞分布在时间和空间在出生后的大脑发育。(C)2004 Wiley-Liss，Inc.
Homer acts as a postsynaptic adaptor protein that links multiple targets, such as proteins involved in glutamate receptor signaling. We report the differential expression of the long form of Homer proteins produced from three distinctive genes during postnatal development of the mouse brain. Homer 1b/c and Cupidin/Homer 2a/b are widespread throughout the developing brain and are down-regulated in hindbrain-origin regions, such as the cerebellum, pons, and medulla oblongata. In contrast, Homer 3a/b is restricted to the cerebellum, hippocampus, and neonatal olfactory bulb. In the cerebellum, Homer 1b/c and Cupidin/Homer 2a/b predominate in the postsynapses of developing granule cells, whereas Homer 3a/b is concentrated in the dendritic spines of Purkinje cells and their axons. The down-regulation of Homer 1b/c and Cupidin/Homer 2a/b is in marked contrast to the up-regulation of Homer 3a/b between the first and the second postnatal weeks. In the hippocampus, Homer 1b/c and Cupidin/Homer 2a/b are largely located in the CA1 region and the CA1-CA2 region, respectively, whereas Homer 3a/b is largely distributed in the CA2-CA3 region and peaks around the third postnatal week. In hippocampal cell cultures, Homer 1b/c and Cupidin/Homer 2a/b are expressed in inhibitory and excitatory neurons, whereas Homer 3a/b is largely expressed in excitatory neurons but not in inhibitory neurons. In the developing olfactory bulb, Homer 1b/c and Cupidin/Homer 2a/b are up-regulated in the granular, external plexiform, and glomerular layers, whereas Homer 3a/b drastically decreases in these regions within the first postnatal week. Cupidin/Homer 2a/b is also expressed in olfactory sensory neurons within a distinct olfactory epithelial zone and is then widely distributed to both the axons in the olfactory nerve layer and the cilia in the olfactory epithelium. These results demonstrate that Homer family members have distinct regional, cellular, and subcellular distributions in time and space during postnatal brain development. (C) 2004 Wiley-Liss, Inc.