R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes).
R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes).
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R-spondins 参与硬骨鱼青鳉 (Oryzias latipes) 的卵巢分化
DOI：
10.1186/1471-213x-12-36
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发表时间：
2012-12-07
影响因子：
--
通讯作者：
Nagahama Y
中科院分区：
文献类型：
--
作者：
Zhou L;Charkraborty T;Yu X;Wu L;Liu G;Mohapatra S;Wang D;Nagahama Y
在哺乳动物中，R-spondin（Rspo），Wnt/β-catenin信号通路的激活剂，已被证明参与卵巢分化。然而，Rspo/Wnt/β-catenin信号通路在鱼类性腺中的作用仍然未知。本研究从青鳉生殖腺中克隆了Rspo1、2和3的全长cDNA。推测的氨基酸序列的mRspo1 - 3被证明有一个类似的结构组织。系统发育分析表明，Rspo1、2和3分别聚为3个不同的分支。组织分布显示，三个Rspo基因在大脑和卵巢中大量表达。孵化前后（S33 - 5dah）的实时荧光定量PCR分析表明，三个Rspo基因在S38的雌性性腺中特异性增强。原位杂交（ISH）结果表明，Rspo基因在卵巢生殖细胞中表达，而在精巢中不表达。多色荧光原位杂交结果显示，Rspo1在10dah时在体细胞和生殖细胞中均有表达。XY个体暴露于乙烯利（EE 2）一周后，在0 dah和成年期均显著增强了三个Rspo基因的表达。这些结果表明，Rspo激活信号通路参与了青鳉卵巢的分化和维持。
In mammals, R-spondin (Rspo), an activator of the Wnt/β-catenin signaling pathway, has been shown to be involved in ovarian differentiation. However, the role of the Rspo/Wnt/β-catenin signaling pathway in fish gonads is still unknown. In the present study, full-length cDNAs of Rspo1, 2 and 3 were cloned from the gonads of medaka (Oryzias latipes). The deduced amino acid sequences of mRspo1-3 were shown to have a similar structural organization. Phylogenetic analysis showed that Rspo1, 2 and 3 were specifically clustered into three distinct clads. Tissue distribution revealed that three Rspo genes were abundantly expressed in the brain and ovary. Real-time PCR analysis around hatching (S33-5dah) demonstrated that three Rspo genes were specifically enhanced in female gonads from S38. In situ hybridization (ISH) analysis demonstrated that three Rspo genes were expressed in the germ cell in ovary, but not in testis. Fluorescence multi-color ISH showed that Rspo1 was expressed in both somatic cells and germ cells at 10dah. Exposure to ethinylestradiol (EE2) in XY individuals for one week dramatically enhanced the expression of three Rspo genes both at 0dah and in adulthood. These results suggest that the Rspo-activating signaling pathway is involved in the ovarian differentiation and maintenance in medaka.