The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo.
The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo.
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DOI：
10.1073/pnas.81.4.1184
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发表时间：
1984-02
影响因子：
11.1
通讯作者：
S. Wong;C. Price;D. Goldfarb;R. Doi
中科院分区：
文献类型：
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作者：
S. Wong;C. Price;D. Goldfarb;R. Doi
一个克隆的枯草芽孢杆菌基因（sprE）只在稳定生长期表达，编码枯草杆菌蛋白酶E蛋白酶，一种与孢子形成相关的酶。我们已经确定了sprE启动子区和启动子近端一半的结构基因的DNA序列。sprE基因编码一个假定的29个残基的信号肽和一个77个残基的前导肽之前的成熟枯草杆菌蛋白酶序列。通过质粒整合和噬菌体PBS 1转导，我们定位了B上gly B和metD之间的sprE位点。枯草杆菌染色体，一个区域也含有超蛋白酶生产hpr基因。在体外，sprE基因由含有37，000-道尔顿σ因子（σ 37）的RNA聚合酶的次要形式转录。我们表明，S1核酸酶映射，sprE转录启动在双起始位点在体外和体内，并为下游网站的启动子具有特征性的西格玛37识别序列。我们提出，西格玛37 RNA聚合酶的生理作用是转录一类基因，这些基因被分解代谢物抑制，编码胞外酶，或仅在生长的稳定期表达。
A cloned Bacillus subtilis gene (sprE) expressed only during the stationary growth phase is shown to encode the subtilisin E protease, an enzyme associated with sporulation. We have determined the DNA sequence of the sprE promoter region and the promoter-proximal half of the structural gene. The sprE gene codes for a putative 29-residue signal peptide and a 77-residue leader peptide preceding the mature subtilisin sequence. By plasmid integration and phage PBS1 transduction, we have mapped the sprE locus between glyB and metD on the B. subtilis chromosome, a region also containing the hyperprotease-producing hpr gene. In vitro the sprE gene is transcribed by the minor form of RNA polymerase containing a 37,000-dalton sigma factor (sigma 37). We show by S1 nuclease mapping that sprE transcription initiates at dual start sites both in vitro and in vivo and that the promoter for the downstream site has a characteristic sigma 37 recognition sequence. We propose that the physiological role of the sigma 37 RNA polymerase is to transcribe a class of genes that are catabolite repressed, that encode extracellular enzymes, or that are expressed only during the stationary phase of growth.