Immunoelectrophoretic Studies on Human Small Intestinal Brush Border Proteins. The Residual Isomaltase in Sucrose Intolerant Patients
Immunoelectrophoretic Studies on Human Small Intestinal Brush Border Proteins. The Residual Isomaltase in Sucrose Intolerant Patients
复制标题
人小肠刷状缘蛋白的免疫电泳研究。
作者：
H. Skovbjerg;P. Krasilnikoff
摘要：对3例蔗糖不耐受（蔗糖酶异麦芽糖酶缺乏症）患者的小肠活检进行了免疫电泳和酶分析。所有患者均缺乏蔗糖酶活性（<1单位/克蛋白）。其中一名患者具有大量的异麦芽糖酶活性（7.8单位/克蛋白质）。免疫电泳显示该患者存在蔗糖-异麦芽糖酶的异麦芽糖酶多肽。所有的活组织检查都没有显示任何可能代表改性的无活性蔗糖酶蛋白的沉淀物。推测：蔗糖不耐受患者可能是一个异质性的群体，一些患者缺乏蔗糖酶和异麦芽糖酶活性，而另一些患者保留了活性的异麦芽糖酶多肽。
Summary: Small intestinal biopsies from three patients with sucrose intolerance (sucrase-isomaltase deficiency) were studied by means of immunoelectrophoresis and enzymatic assays. All patients lacked sucrase activity (<1 unit/g protein). One of the patients had a substantial isomaltase activity (7.8 unit/g protein). Immunoelectrophoresis revealed the presence of the isomaltase polypeptide of the sucrase-isomaltase in this patient. None of the biopsies showed any precipitate that might represent a modified inactive sucrase protein.Speculation: It is probable that patients with sucrose intolerance constitute a heterogeneous group, with some of the patients lacking both sucrase and isomaltase activities whereas others have retained an active isomaltase polypeptide.