Anaerobic ammonium oxidation and denitrification in a paddy soil as affected by temperature, pH, organic carbon, and substrates
Anaerobic ammonium oxidation and denitrification in a paddy soil as affected by temperature, pH, organic carbon, and substrates
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DOI：
10.1007/s00374-018-1263-z
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发表时间：
2018-01
影响因子：
6.5
通讯作者：
J. Shan;Pinpin Yang;X. Shang;M. M. Rahman-M.;M. M. Rahman-M.;Xiaoyuan Yan
中科院分区：
文献类型：
--
作者：
J. Shan;Pinpin Yang;X. Shang;M. M. Rahman-M.;M. M. Rahman-M.;Xiaoyuan Yan
厌氧氨氧化（厌氧氨氧化过程）广泛发生在水稻土中，并可能大大有助于永久去除氮，但很少有人知道控制这个过程的因素。采用培养法结合15 N示踪技术，研究了温度、pH、有机碳和基质对稻田土壤厌氧氨氧化潜力的影响，以及厌氧氨氧化对稻田土壤总N2产生的相对贡献。厌氧氨氧化的温度范围为5 - 35 °C，最适速率为25 °C（1.7 nmol N g−1h−1），pH范围为4.8 - 10.1，最适速率为pH 7.3（1.7 nmol N g−1h−1）。葡萄糖和乙酸盐（5-100 mg C L−1）的存在显著抑制了厌氧氨氧化活性和厌氧氨氧化与总N2产量的比率。厌氧氨氧化的潜在速率对铵浓度的响应符合米氏关系，最大速率（Vmax）为4.4 nmol N g−1h− 1，亲和常数（Km）为6.3 mg NH 4 +-N L−1。硝酸盐（5-15 mg 15 NO3--N L-1）显著抑制厌氧氨氧化酶活性和厌氧氨氧化酶占总N2产量的比例。研究结果为分析稻田土壤厌氧氨氧化过程的控制因素及其对氮素流失的贡献提供了有益的信息。
Anaerobic ammonium oxidation (anammox process) widely occurs in paddy soil and may substantially contribute to permanent N removal; however, little is known about the factors controlling this process. Here, effects of temperature, pH, organic C, and substrates on potential rate of anammox and the relative contribution of anammox to total N2production in a paddy soil were investigated via slurry incubation combined with15N tracer technique. Anammox occurred over a temperature range from 5 to 35 °C with an optimum rate at 25 °C (1.7 nmol N g−1h−1) and a pH range from 4.8 to 10.1 with an optimum rate at pH 7.3 (1.7 nmol N g−1h−1). The presence of glucose and acetate (5–100 mg C L−1) significantly inhibited anammox activities and the ratio of anammox to total N2production. The response of potential rates of anammox to ammonium concentrations fitted well with Michaelis-Menten relationship showing a maximum rate (Vmax) of 4.4 nmol N g−1h−1and an affinity constant (Km) of 6.3 mg NH4+-N L−1. Whereas, nitrate addition (5–15 mg15NO3−-N L−1) significantly inhibited anammox activities and the ratio of anammox to total N2production. Our results provide useful information on factors controlling anammox process and its contribution to N loss in the paddy soil.