Preparation and biological evaluations of a collagen-like hierarchical Ti surface with superior osteogenic capabilities
Preparation and biological evaluations of a collagen-like hierarchical Ti surface with superior osteogenic capabilities
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具有优异成骨能力的类胶原分层钛表面的制备和生物学评价
DOI：
10.1039/d0tb00799d
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发表时间：
2020
影响因子：
7
通讯作者：
Li Xiaodong
中科院分区：
文献类型：
--
作者：
Lao Weiwei;Luo Qiaojie;Chen Yadong;Yao Wei;Xu Jiajia;Fan Lijie;Li Xiaodong
高成骨能力的多尺度钛表面的构建一直在口腔种植和植入生物材料领域引起广泛关注。然而，迄今为止，对多尺度表面结构与生物学特性之间的相关性缺乏深入的了解是这些多尺度植入物开发的主要障碍。在这项研究中，通过三步减成法制备了一系列新型多尺度钛表面。此外，基于SEM图像的灰度分析，我们开发了多尺度表面形貌分析方法。可以根据相应的放大SEM图像来分析多尺度复杂表面的每个尺度的典型形貌特征。因此，可以用数学方法描述表面形貌从简单表面到多尺度复杂表面的演化规律。在此基础上，建立了多尺度表面结构与相应生物学特性之间的相关性。对于具有优异成骨能力的多尺度表面，在多个尺度（即多尺度有序）的结构之间发现了严格的内在规律性，即3D类胶原网络纳米结构的构建与经典微粗糙表面预先存在的宏观和微观结构的典型形貌特征的保留之间存在平衡。此外，进一步发现多尺度有序的分层Ti表面结构可以调节ROS的产生并增强巨噬细胞M2极化，从而创造有利于成骨的免疫炎症微环境，并协同地表现出优异的生物学能力。因此，获得了具有优异成骨能力的优化的类胶原蛋白分层表面。
The construction of multiscale Ti surfaces of high osteogenic ability has always attracted significant attention in the fields of oral implantology and implantable biomaterials. However, to date, the absence of a solid understanding of the correlation between the multiscale surface structure and the biological properties is the main obstacle in the development of these multiscale implants. In this study, a series of novel multiscale Ti surfaces were prepared via a three-step subtractive method. Moreover, based on the grayscale analysis of SEM images, we developed multiscale surface topography analysis methods. The typical topography characteristics at each scale of a multiscale complex surface can be analyzed according to the corresponding magnified SEM images. Thus, the evolution rule of the surface topography from a simple surface to multiscale complex surfaces can be mathematically described. Based on this, the correlation between multiscale surface structures and the corresponding biological properties was established. For the multiscale surface of superior osteogenic capacity, strict inherent regularity was found among the structures at multiple scales (i.e., multiscale order), that is, there was a balance between the construction of the 3D collagen-like network nanostructure and the preservation of the typical topographical features of the pre-existing macro- and micro-structures of the classic micro-roughened surface. Moreover, it was further found that the multiscale-ordered hierarchical Ti surface structure could modulate ROS production and enhance macrophage M2 polarization to create an osteogenesis-favorable immuno-inflammatory microenvironment and synergistically exhibit superior biological capability. Consequently, an optimized collagen-like hierarchical surface with superior osteogenic abilities was achieved.