Microfluidic system with integrated electroosmotic pumps, concentration gradient generator and fish cell line (RTgill-W1)-towards water toxicity testing
Microfluidic system with integrated electroosmotic pumps, concentration gradient generator and fish cell line (RTgill-W1)-towards water toxicity testing
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DOI：
10.1039/b911412m
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Ren, Carolyn L.
中科院分区：
文献类型：
--
作者：
Glawdel, Tomasz;Elbuken, Caglar;Ren, Carolyn L.
本研究提出了一种微流体系统，该系统结合了电渗泵、浓度梯度发生器和鱼细胞系（虹鳟鱼鳃），可对系统中接种的鱼细胞进行毒性测试。该系统由三个机械组件组成：(1) 毒性测试芯片，包含微流体梯度发生器，可在细胞测试室中产生毒物的线性浓度分布；(2) 电渗 (EO) 泵芯片，控制毒性芯片的流速和操作；(3) 连接两个芯片的间接储液器，允许泵送毒物溶液 与电渗泵溶液分开。通过使用荧光显微镜监测梯度发生器来测量和测试 EO 泵的流量和稳定性。此外，使用虹鳟鱼鳃细胞系（RTgill-W1）作为测试细胞并使用十二烷基硫酸钠作为模型毒物，通过该系统设置进行了致死率测试。将十二烷基硫酸钠梯度（从0至50μg mL(-1)）施加到贴壁细胞上1小时，并使用Live/Dead TM 细胞测定对结果进行定量。这项工作是对 EO 泵在活细胞测定中应用的初步研究，有可能在以 RTgill-W1 细胞作为生物传感器的便携式水质检测设备中使用该泵。
This study presents a microfluidic system that incorporates electroosmotic pumps, a concentration gradient generator and a fish cell line (rainbow trout gill) to perform toxicity testing on fish cells seeded in the system. The system consists of three mechanical components: (1) a toxicity testing chip containing a microfluidic gradient generator which creates a linear concentration distribution of toxicant in a cell test chamber, (2) an electroosmotic (EO) pump chip that controls the flow rate and operation of the toxicity chip, and (3) indirect reservoirs that connect the two chips allowing for the toxicant solution to be pumped separately from the electroosmotic pump solution. The flow rate and stability of the EO pumps was measured and tested by monitoring the gradient generator using fluorescence microscopy. Furthermore, a lethality test was performed with this system setup using a rainbow trout gill cell line (RTgill-W1) as the test cells and sodium dodecyl sulfate as a model toxicant. A gradient of sodium dodecyl sulfate, from 0 to 50 mu g mL(-1), was applied for 1 hr to the attached cells, and the results were quantified using a Live/Dead(TM) cell assay. This work is a preliminary study on the application of EO pumps in a living cell assay, with the potential to use the pumps in portable water quality testing devices with RTgill-W1 cells as the biosensors.