A gene essential for viability and flagellar regeneration maps to the uni linkage group of Chlamydomonas reinhardtii.
A gene essential for viability and flagellar regeneration maps to the uni linkage group of Chlamydomonas reinhardtii.
复制标题
对活力和鞭毛再生至关重要的基因映射到莱茵衣藻的单连锁群。
DOI：
10.1007/bf00419919
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发表时间：
1989
期刊：
影响因子：
2.5
通讯作者：
Silflow,CD
中科院分区：
文献类型：
--
作者：
Larkin,JC;Lefebvre,PA;Silflow,CD
我们已经分离出一个突变体的衣原体reinhardtii，这是温度敏感的活力和温度敏感的鞭毛再生。的突变（指定tnr1，为温度敏感的onreactor）已被遗传映射到一个位置接近uni1上的unilinkage组（ULG），一个不寻常的遗传环状连锁群，主要由影响鞭毛组装或功能的突变。tnr1是第一个必需的基因确定在这个连锁群，是为数不多的必需基因影响鞭毛功能确定的日期。我们还发现，thattnr1细胞是没有缺陷的诱导新的微管蛋白转录或蛋白质合成过程中鞭毛再生在非允许的温度，和至少有一部分的未组装池的鞭毛蛋白在突变体细胞的组装能力在非允许的温度。
We have isolated a mutant ofChlamydomonas reinhardtiithat is both temperature sensitive for viability and temperature sensitive for flagellar regeneration. The mutation (designatedtnr1, fortemperature-sensitivenonregenerator) has been genetically mapped to a position nearuni1on theunilinkage group (ULG), an unusual genetically circular linkage group consisting primarily of mutations affecting flagellar assembly or function.tnr1is the first essential gene identified on this linkage group, and is one of the few essential genes affecting flagellar function identified to date. We also find thattnr1cells are not defective for induction of new tubulin transcripts or protein synthesis during flagellar regeneration at the nonpermissive temperature, and that at least a portion of the unassembled pool of flagellar proteins in mutant cells is assembly-competent at the nonpermissive temperature.