Activation of transgene expression in skeletal muscle by focused ultrasound.
Activation of transgene expression in skeletal muscle by focused ultrasound.
复制标题
通过聚焦超声激活骨骼肌中的转基因表达。
DOI：
10.1016/j.bbrc.2008.12.090
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发表时间：
2009
影响因子：
3.1
通讯作者：
Zhong,P
中科院分区：
文献类型：
--
作者：
Lu,X;Sankin,G;Pua,EC;Madden,J;Zhong,P
为了将聚焦超声热剂量与基因表达和组织损伤联系起来，采用了温度平台策略。在hsp70B启动子控制下，编码荧光素酶基因的质粒通过电穿孔转染大鼠右侧腓肠肌。转染后第1天，后肢接受3.3 mhz聚焦超声治疗，采用4个不同温度平台，分别为46°C、48°C、51°C和62°C。高原温度下处理时间为0 ~ 30s。在FUS治疗1天后分析基因在体内的表达，并用H&E染色评估组织损伤。基因激活和组织损伤与热剂量密切相关。在TSPTA=51°C下，FUS诱导的基因激活水平最高，持续20s，与水浴热疗产生的基因激活水平在统计学上相当。
To correlate thermal dose from focused ultrasound (FUS) with gene expression and tissue injury, a temperature plateau strategy was employed. Plasmids encoding luciferase gene under the control of hsp70B promoter were transfected into the right gastrocnemius muscle in a rat via electroporation. One day after transfection, hind limbs were treated with 3.3-MHz focused ultrasound, using one of four different temperature plateaus with spatial-peak time-average focal temperatures (TSPTA) of 46°C, 48°C, 51°C and 62°C. The treatment duration at the plateau temperature was varied from 0 to 30s. Gene expression was analyzed in vivo one day following FUS treatment, and H&E staining was employed to assess tissue injury. Gene activation and tissue damage correlated closely with thermal dose. The highest level of gene activation was induced by FUS at TSPTA=51°C for 20s, which was found to be statistically equivalent to that produced by water-bath hyperthermia.