Restricted expression of the homeobox gene prox 1 in developing zebrafish
Restricted expression of the homeobox gene prox 1 in developing zebrafish
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DOI：
10.1016/s0925-4773(98)00121-x
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发表时间：
1998-08-01
影响因子：
2.6
通讯作者：
Tomarev, SI
中科院分区：
文献类型：
--
作者：
Glasgow, E;Tomarev, SI
Prox 1是脊椎动物的同源异型盒基因，与果蝇的转录因子Proxo同源.我们已经从斑马鱼中分离出一个prox 1 cDNA，它编码的蛋白质与鸡、小鼠和人的Prox 1分别具有82%、84%和83%的氨基酸同源性。针对人Prox 1提出的抗体与斑马鱼Prox 1交叉反应，并在这里用于通过整装免疫组织化学确定斑马鱼胚胎发生过程中Prox 1的表达模式。在10体节胚胎中，Prox 1在预期的透镜基板和从眼睛延伸到耳泡的广泛上皮区域上表达。随着胚胎发生的进行，眼透镜中的Prox 1表达变得强烈，并在成熟的肌肉先锋细胞和表面肌肉细胞中检测到。在CNS中，Prox 1以沿着前脑-中脑边界的条纹形式表达，在腹侧后脑中以分段模式表达，并且在腹侧脊髓的选定细胞中表达。Prox 1表达的其他位点包括侧线原基、三叉神经节、耳泡和偶尔的内胚层细胞。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Prox 1 is a vertebrate homeobox gene which is homologous to the Drosophila transcription factor, prospero. We have isolated a prox 1 cDNA from zebrafish, which encodes a protein that has 82%, 84% and 83% amino acid identity with chicken, mouse and human Prox 1, respectively. Antibodies raised against human Prox 1 cross-react with zebrafish Prox 1 and are used here to determine the expression patterns of Prox 1 during zebrafish embryogenesis by whole-mount immunohistochemistry. In the 10-somite embryo, Prox 1 is expressed over the prospective lens placode and over a broad region of epithelium extending from the eye to the otic vesicle. As embryogenesis proceeds, Prox 1 expression in the eye lens becomes intense, and is detected in maturing muscle pioneer cells and superficial muscle cells. In the CNS, Prox 1 is expressed in a stripe along the forebrain-midbrain boundary, in a segmented pattern in the ventral hindbrain, and in selected cells of the ventral spinal cord. Additional sites of Prox 1 expression include the lateral line primordium, the trigeminal ganglia, the otic vesicle and occasional endodermal cells. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.