Identification of laminin α1 and β1 chain peptides active for endothelial cell adhesion, tube formation, and aortic sprouting
Identification of laminin α1 and β1 chain peptides active for endothelial cell adhesion, tube formation, and aortic sprouting
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DOI：
10.1096/fasebj.13.1.53
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发表时间：
1999-01-01
期刊：
影响因子：
4.8
通讯作者：
Ponce, ML
中科院分区：
文献类型：
--
作者：
Malinda, KM;Nomizu, M;Ponce, ML
层粘连蛋白-1是一种基底膜糖蛋白，促进多种生物活性，包括细胞附着、肿瘤转移和血管生成。血管生成在组织形成、繁殖、创面愈合等方面起着重要作用。有几种病理情况。在这项研究中，我们从al链和pi链中筛选了405个合成肽，以确定内皮细胞对层粘连蛋白- 1有活性的潜在位点。肽最初是通过测试内皮细胞对肽包被孔的粘附以及可溶性肽存在时在基质上形成管的情况来筛选的。利用大鼠主动脉环发芽实验进行二次筛选，鉴定出20种肽中的13种刺激内皮细胞发芽，其中一些活性肽也在Boyden室实验中被发现刺激人脐静脉内皮细胞迁移。在所有的实验中，观察到反应所需的肽的数量和所产生的形态/反应的差异。我们的研究结果表明，层粘胶蛋白-1上的几个活性结构域可能在刺激血管生成的不同步骤中起重要作用。
Laminin-1 is a basement membrane glycoprotein that promotes several biological activities including cell attachment, tumor metastasis, and angiogenesis. Angiogenesis plays an important role in tissue formation, reproduction, wound healing,;md several pathological conditions. In this study, we screened 405 synthetic peptides from the al and pi chains to identify potential sites on laminin-l active with endothelial cells, Peptides were initially screened by testing both endothelial cell adhesion to peptide-coated wells and tube formation on Matrigel in the presence of soluble peptide. Twenty active peptides were identified in these screens, A secondary screen using the rat aortic ring sprouting assay identified 13 of the 20 peptides that stimulated endothelial sprouting, Several of these active peptides were also found to stimulate human umbilical vein endothelial cell migration in Boyden chamber assays. Differences in the amount of peptide needed for the response and in the resultant morphologies/responses were observed between the peptides in all of the assays. Our results suggest that several active domains on laminin-1 may play important roles in stimulating different steps in angiogenesis.