Complex expression dynamics and robustness in C. elegans insulin networks
Complex expression dynamics and robustness in C. elegans insulin networks
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DOI：
10.1101/gr.150466.112
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发表时间：
2013-06-01
期刊：
影响因子：
7
通讯作者：
Walhout, Albertha J. M.
中科院分区：
文献类型：
--
作者：
Ritter, Ashlyn D.;Shen, Yuan;Walhout, Albertha J. M.
基因家族通过基因复制而扩大，并通过突变产生相似的分支。功能多样化包括对原有功能的新功能化和亚功能化。此外，多个基因完成重叠功能的冗余性经常被维持。在这里，我们使用40个秀丽隐杆线虫胰岛素家族来深入了解特异性和冗余之间的平衡。胰岛素/胰岛素样生长因子（IIS）途径由单一受体DAF-2组成。迄今为止，没有单一的胰岛素样肽概括所有daf -2相关表型，可能是由于胰岛素样基因之间的冗余。为了提供潜在冗余模式的第一级注释，我们全面描述了所有40种胰岛素在活体动物中的时空和条件表达。我们在表达中观察到广泛的动态，这可以解释缺乏简单的两两冗余模式。我们提出了一个模型，其中基因家族进化，以获得不同的联盟在不同的组织和响应一系列的环境压力。
Gene families expand by gene duplication, and resulting paralogs diverge through mutation. Functional diversification can include neofunctionalization as well as subfunctionalization of ancestral functions. In addition, redundancy in which multiple genes fulfill overlapping functions is often maintained. Here, we use the family of 40 Caenorhabditis elegans insulins to gain insight into the balance between specificity and redundancy. The insulin/insulin-like growth factor (IIS) pathway comprises a single receptor, DAF-2. To date, no single insulin-like peptide recapitulates all DAF-2-associated phenotypes, likely due to redundancy between insulin-like genes. To provide a first-level annotation of potential patterns of redundancy, we comprehensively delineate the spatiotemporal and conditional expression of all 40 insulins in living animals. We observe extensive dynamics in expression that can explain the lack of simple patterns of pairwise redundancy. We propose a model in which gene families evolve to attain differential alliances in different tissues and in response to a range of environmental stresses.