DNA-binding chromosomal non-histone proteins. Isolation, characterization, and tissue specifity.
DNA-binding chromosomal non-histone proteins. Isolation, characterization, and tissue specifity.
复制标题
DNA 结合染色体非组蛋白。
DOI：
10.1021/bi00691a034
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发表时间：
1975
期刊：
影响因子：
2.9
通讯作者：
L. Hnilica
中科院分区：
文献类型：
--
作者：
J. Chiu;S. Wang;H. Fujitani;L. Hnilica
A fractionation schedule is described which allows the isolation of a group of chromosomal non-histone proteins (NP) with affinity for DNA. In polyacrylamide gel electrophoresis these proteins isolated from rat liver are represented principally by a group of low molecular weight polypeptides. The NP fraction comprises about 2-4% of the total chromatin protein content in rat liver or Novikoff hepatoma. Experiments in vivo and in vitro revealed that the NP proteins do not incorporate significant amounts of 32P. Complexes of the chromosomal proteins NP with homologous DNA are immunologically tissue specific and the specificity can be transferred by reconstituting the NP proteins from one tissue to the residual chromatin from another.
