Reproductive organ dysfunction and gene expression after orally administration of ZnO nanoparticles in murine
Reproductive organ dysfunction and gene expression after orally administration of ZnO nanoparticles in murine
复制标题
口服氧化锌纳米颗粒后小鼠生殖器官功能障碍和基因表达。
DOI：
10.1002/tox.23060
复制
发表时间：
2020-11-25
影响因子：
4.5
通讯作者：
Xu, Hengyi
中科院分区：
文献类型：
--
作者：
Kuang, Huijuan;Zhang, Wanyi;Xu, Hengyi
ZnO纳米颗粒（NPs）是消费品、工业和研究中制造最多的纳米颗粒之一。越来越多的证据表明ZnO纳米颗粒在体内表现出毒理学效应。氧化锌纳米颗粒毒性的性别差异尚不清楚，因此本研究的目的是研究氧化锌纳米颗粒对雌性和雄性生殖器官（子宫，卵巢和睾丸）的影响。将ZnO纳米颗粒以0和100 mg/kg体重的剂量水平口服施用给雌性和雄性小鼠。在管饲后3天对生物材料取样。结果表明，锌在处理小鼠的生殖器官中积累。此外，ZnO纳米颗粒给药诱导雄性小鼠睾丸重量显著下降，血液学和血清生化参数失衡。组织病理学检查显示，ZnO NPs染毒小鼠出现结构紊乱、细胞凋亡和死亡。此外，RT-qPCR数据表明ZnO纳米颗粒可以激活子宫介导的信号通路并诱导caspase依赖性损伤，最终损伤子宫。在卵巢中，ZnO纳米颗粒诱导Shh通路激活的卵巢细胞凋亡，并影响类固醇合成。在睾丸中，ZnO纳米颗粒有效地改变了与氧化应激、排毒/代谢过程和细胞凋亡相关的基因的表达水平。结果表明，氧化锌纳米颗粒对雄性小鼠的生殖毒性大于雌性小鼠。总体而言，这些研究结果表明，氧化锌纳米颗粒可能会引起与生殖健康相关的风险，特别是在那些谁是在职业水平。
ZnO nanoparticles (NPs) are among the most manufactured nanoparticles in the consumer products, industries, and researches. An increasing body of evidence indicated that ZnO NPs show toxicological effects in vivo. Sex differences in the toxicity of ZnO NPs are not clear, thus the aim of this study was to investigate the effects of ZnO NPs on the female and male reproductive organs (uterus, ovary and testes). ZnO NPs were orally administered to female and male mice at dosages level of 0 and 100 mg/kg body weight. The biological material was sampled 3 days after tube feeding. The results demonstrated that Zinc contents were accumulated in the reproductive organs of treated mice. Furthermore, ZnO NPs administration induced significant decrease in the testes weight, an imbalance of hematological and serum biochemical parameters in male mice. The histopathological examinations showed that structural disorder and the appearance of cell apoptosis and death in the ZnO NPs-exposed mice. Additionally, the RT-qPCR data indicated ZnO NPs can activate mitochondrial-mediated signaling pathway and induce caspase depend damage that ultimately injured the uterus. In the ovary, ZnO NPs induce cell apoptosis in Shh pathway activated ovary cells, and affect the synthesis of steroidogenesis. In the testes, ZnO NPs effectively changed the expression level of genes related to oxidant stress, detox/metabolic process, and apoptosis. It was found that ZnO NPs caused more serious reproductive toxicity in the male mice than female mice. Overall, these findings indicated that ZnO NPs could induce exposure-related risks to reproductive health, especially in those who are at the occupational level.